Human GSDMD ELISA Kit

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  • Alternative name

    Human gasdermin domain-containing protein 1 ELISA Kit;Human GSDMD-NT ELISA Kit;Human GSDMD-CT ELISA Kit;Human DFNA5L ELISA Kit;Human GSDMDC1 ELISA Kit;Human DF5L ELISA Kit;Human FKSG10 ELISA Kit;Human gasdermin D ELISA Kit;Human gasdermin-D ELISA Kit;Human gasdermin domain containing 1 ELISA Kit;

  • Catalog
    E016177
  • species
    Human
  • GeneGSDMD
  • Standard CurveHuman GSDMD ELISA Kit
  • Other Species Mouse GSDMD ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GSDMD. No significant cross-reactivity or interference between GSDMD and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.122ng/mL.
  • Intended UseHuman GSDMD ELISA Kit allows for the in vitro quantitative determination of GSDMD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GSDMD in human tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSDMD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GSDMD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSDMD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GSDMD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Gasdermin-D, C-terminal Protein information
  • Uniprot ID GSDMD_HUMAN
  • Uniprot AC P57764; A8K702; D3DWJ9; Q96Q98;
  • UniGene Hs.118983;
  • GeneID 79792
  • KEGG hsa:79792;
  • Human Gasdermin-D, C-terminal Protein SEQUENCE
  • SEQUENCE 484 AA; 52801 MW; F7CE8073E0C0194D CRC64;

    MGSAFERVVR RVVQELDHGG EFIPVTSLQS STGFQPYCLV VRKPSSSWFW

    KPRYKCVNLS IKDILEPDAA EPDVQRGRSF HFYDAMDGQI QGSVELAAPG

    QAKIAGGAAV SDSSSTSMNV YSLSVDPNTW QTLLHERHLR QPEHKVLQQL

    RSRGDNVYVV TEVLQTQKEV EVTRTHKREG SGRFSLPGAT CLQGEGQGHL

    SQKKTVTIPS GSTLAFRVAQ LVIDSDLDVL LFPDKKQRTF QPPATGHKRS

    TSEGAWPQLP SGLSMMRCLH NFLTDGVPAE GAFTEDFQGL RAEVETISKE

    LELLDRELCQ LLLEGLEGVL RDQLALRALE EALEQGQSLG PVEPLDGPAG

    AVLECLVLSS GMLVPELAIP VVYLLGALTM LSETQHKLLA EALESQTLLG

    PLELVGSLLE QSAPWQERST MSLPPGLLGN SWGEGAPAWV LLDECGLELG

    EDTPHVCWEP QAQGRMCALY ASLALLSGLS QEPH

  • UCSC uc003yyg.4; human.;


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