Human GJC1 ELISA Kit

Alternative name
Human connexin-45 ELISA Kit;Human CX45 ELISA Kit;Human gap junction alpha-7 protein ELISA Kit;Human GJA7 ELISA Kit;Human gap junction protein gamma 1 ELISA Kit;Human gap junction gamma-1 protein ELISA Kit;Human CTC-296K1.4 ELISA Kit;Human gap junction protein, gamma 1, 45kDa ELISA Kit;
Catalog
E016142
species
Human
GeneGJC1
SpecificityThis assay has high sensitivity and excellent specificity for detection of GJC1. No significant cross-reactivity or interference between GJC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GJC1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman GJC1 ELISA Kit allows for the in vitro quantitative determination of GJC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This GJC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GJC1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GJC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GJC1 antibody and an GJC1-HRP conjugate. The assay sample and buffer are incubated together with GJC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GJC1 concentration since GJC1 from samples and GJC1-HRP conjugate compete for the anti-GJC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GJC1 from the sample, fewer sites are left to bind GJC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GJC1 concentration in each sample is interpolated from this standard curve.
Human Gap junction gamma-1 protein Protein information
Uniprot ID CXG1_HUMAN
Uniprot AC P36383; B3KW68; Q4VAY0;
UniGene Hs.712052; Hs.744748;
GeneID 10052
KEGG hsa:10052;
Human Gap junction gamma-1 protein Protein SEQUENCE
SEQUENCE 396 AA; 45470 MW; C9F67C1D926D40DF CRC64;
MSWSFLTRLL EEIHNHSTFV GKIWLTVLIV FRIVLTAVGG ESIYYDEQSK
FVCNTEQPGC ENVCYDAFAP LSHVRFWVFQ IILVATPSVM YLGYAIHKIA
KMEHGEADKK AARSKPYAMR WKQHRALEET EEDNEEDPMM YPEMELESDK
ENKEQSQPKP KHDGRRRIRE DGLMKIYVLQ LLARTVFEVG FLIGQYFLYG
FQVHPFYVCS RLPCPHKIDC FISRPTEKTI FLLIMYGVTG LCLLLNIWEM
LHLGFGTIRD SLNSKRRELE DPGAYNYPFT WNTPSAPPGY NIAVKPDQIQ
YTELSNAKIA YKQNKANTAQ EQQYGSHEEN LPADLEALQR EIRMAQERLD
LAVQAYSHQN NPHGPREKKA KVGSKAGSNK STASSKSGDG KTSVWI
UCSC uc002ihj.4; human.;

Category

Human GJC1 ELISA Kit

Human connexin-45 ELISA Kit;Human CX45 ELISA Kit;Human gap junction alpha-7 protein ELISA Kit;Human GJA7 ELISA Kit;Human gap junction protein gamma 1 ELISA Kit;Human gap junction gamma-1 protein ELISA Kit;Human CTC-296K1.4 ELISA Kit;Human gap junction protein, gamma 1, 45kDa ELISA Kit;