Human GJC1 ELISA Kit

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  • Alternative name

    Human connexin-45 ELISA Kit;Human CX45 ELISA Kit;Human gap junction alpha-7 protein ELISA Kit;Human GJA7 ELISA Kit;Human gap junction protein gamma 1 ELISA Kit;Human gap junction gamma-1 protein ELISA Kit;Human CTC-296K1.4 ELISA Kit;Human gap junction protein, gamma 1, 45kDa ELISA Kit;

  • Catalog
    E016142
  • species
    Human
  • GeneGJC1
  • Standard CurveHuman GJC1 ELISA Kit
  • Other Species Mouse Gjc1 ELISA KitBovine GJC1 ELISA KitCanine GJC1 ELISA KitChicken GJC1 ELISA KitHamster GJC1 ELISA KitPorcine GJC1 ELISA KitRat Gjc1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GJC1. No significant cross-reactivity or interference between GJC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GJC1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman GJC1 ELISA Kit allows for the in vitro quantitative determination of GJC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This GJC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GJC1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GJC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GJC1 antibody and an GJC1-HRP conjugate. The assay sample and buffer are incubated together with GJC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GJC1 concentration since GJC1 from samples and GJC1-HRP conjugate compete for the anti-GJC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GJC1 from the sample, fewer sites are left to bind GJC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GJC1 concentration in each sample is interpolated from this standard curve.
  • Human Gap junction gamma-1 protein Protein information
  • Uniprot ID CXG1_HUMAN
  • Uniprot AC P36383; B3KW68; Q4VAY0;
  • UniGene Hs.712052; Hs.744748;
  • GeneID 10052
  • KEGG hsa:10052;
  • Human Gap junction gamma-1 protein Protein SEQUENCE
  • SEQUENCE 396 AA; 45470 MW; C9F67C1D926D40DF CRC64;

    MSWSFLTRLL EEIHNHSTFV GKIWLTVLIV FRIVLTAVGG ESIYYDEQSK

    FVCNTEQPGC ENVCYDAFAP LSHVRFWVFQ IILVATPSVM YLGYAIHKIA

    KMEHGEADKK AARSKPYAMR WKQHRALEET EEDNEEDPMM YPEMELESDK

    ENKEQSQPKP KHDGRRRIRE DGLMKIYVLQ LLARTVFEVG FLIGQYFLYG

    FQVHPFYVCS RLPCPHKIDC FISRPTEKTI FLLIMYGVTG LCLLLNIWEM

    LHLGFGTIRD SLNSKRRELE DPGAYNYPFT WNTPSAPPGY NIAVKPDQIQ

    YTELSNAKIA YKQNKANTAQ EQQYGSHEEN LPADLEALQR EIRMAQERLD

    LAVQAYSHQN NPHGPREKKA KVGSKAGSNK STASSKSGDG KTSVWI

  • UCSC uc002ihj.4; human.;



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