Intended UseHuman AMY1A ELISA Kit allows for the in vitro quantitative determination of AMY1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Alpha-amylase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-amylase 1, During the reaction, Alpha-amylase 1 in the sample or standard competes with a fixed amount of biotin-labeled Alpha-amylase 1 for sites on a pre-coated Monoclonal antibody specific to Alpha-amylase 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Alpha-amylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.