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  • Alternative name

    Alpha-amylase 1 ELISA KIT; 1,4-alpha-D-glucan glucanohydrolase 1 ELISA KIT; Salivary alpha-amylaseAMY1A ELISA KIT; AMY1 ELISA KIT

  • Catalog
  • species
  • GeneAMY1A
  • Standard CurveHuman AMY1A ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural Human Alpha-amylase 1. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity6.20 U/L.
  • Intended UseHuman AMY1A ELISA Kit allows for the in vitro quantitative determination of AMY1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Alpha-amylase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-amylase 1, During the reaction, Alpha-amylase 1 in the sample or standard competes with a fixed amount of biotin-labeled Alpha-amylase 1 for sites on a pre-coated Monoclonal antibody specific to Alpha-amylase 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Alpha-amylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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