Human LGALS4 ELISA Kit

Alternative name
Human gal-4 ELISA Kit;Human antigen NY-CO-27 ELISA Kit;Human L-36 lactose-binding protein ELISA Kit;Human L36LBP ELISA Kit;Human lactose-binding lectin 4 ELISA Kit;Human GAL4 ELISA Kit;Human galectin 4 ELISA Kit;Human galectin-4 ELISA Kit;Human lectin, galactoside-binding, soluble, 4 ELISA Kit;
Catalog
E016018
species
Human
GeneLGALS4
Other Species Mouse Lgals4 ELISA Kit Bovine LGALS4 ELISA Kit Porcine LGALS4 ELISA Kit Rat Lgals4 ELISA Kit
SpecificityNatural and recombinant human Galectin-4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman LGALS4 ELISA Kit allows for the in vitro quantitative determination of LGALS4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Galectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been precoated onto 96-well plates. Standards(E.coli, A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-4 amount of sample captured in plate. Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
Human Galectin-4 Protein information
Uniprot ID LEG4_HUMAN
Uniprot AC P56470;
UniGene Hs.5302;
GeneID 3960
KEGG hsa:3960;
Human Galectin-4 Protein SEQUENCE
SEQUENCE 323 AA; 35941 MW; E79EC0A9AB3990EF CRC64;
MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF
VVGQDPGSDV AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA
FELVFIVLAE HYKVVVNGNP FYEYGHRLPL QMVTHLQVDG DLQLQSINFI
GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP TMEGPPTFNP PVPYFGRLQG
GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR MGNGTVVRNS
LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR
LSAFQRVDTL EIQGDVTLSY VQI

Category

Human LGALS4 ELISA Kit

Human gal-4 ELISA Kit;Human antigen NY-CO-27 ELISA Kit;Human L-36 lactose-binding protein ELISA Kit;Human L36LBP ELISA Kit;Human lactose-binding lectin 4 ELISA Kit;Human GAL4 ELISA Kit;Human galectin 4 ELISA Kit;Human galectin-4 ELISA Kit;Human lectin, galactoside-binding, soluble, 4 ELISA Kit;