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Alternative name
Alpha-actinin-4 ELISA KIT; F-actin cross-linking protein ELISA KIT; Non-muscle alpha-actinin 4ACTN4 ELISA KIT;
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Catalog
E001600
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species
Human
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GeneACTN-4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ACTN-4. No significant cross-reactivity or interference between Human ACTN-4 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe limit of detection of Human ACTN-4 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 19.5 pg/mL (mean of 6 independent assays).
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Intended UseHuman ACTN-4 ELISA Kit allows for the in vitro quantitative determination of ACTN-4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs a two-site sandwich ELISA to quantitative ACTN-4 in Human serum, plasma. An antibody specific for ACTN-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTN-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for ACTN-4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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