Intended UseHuman GAL12 ELISA Kit allows for the in vitro quantitative determination of GAL12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAL12 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAL12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GAL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GAL12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.