Human GAL1 ELISA Kit

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  • Alternative name

    Galectin-1 ELISA KIT; 14 kDa laminin-binding protein ELISA KIT; HLBP14 ELISA KIT; 14 kDa lectin ELISA KIT; Beta-galactoside-binding lectin L-14-I ELISA KIT; Galaptin ELISA KIT; HBL ELISA KIT; HPL ELISA KIT; Lactose-binding lectin 1 ELISA KIT; Lectin galactoside-binding soluble 1 ELISA KIT; Putative MAPK-activating protein PM12 ELISA KIT; S-Lac lectin 1LGALS1 ELISA KIT; Gal-1 ELISA KIT; HLBP14 ELISA KIT

  • Catalog
  • species
  • GeneGAL1
  • Standard CurveHuman GAL1 ELISA Kit
  • Other Species Mouse GAL1 ELISA KitChicken GAL1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GAL1 ELISA Kit allows for the in vitro quantitative determination of GAL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This GAL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GAL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY GAL1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GAL1. Standards or samples are then added to the microtiter plate wells and GAL1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GAL1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GAL1 are added to each well to ?sandwich? the GAL1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GAL1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL1 concentration in each sample is interpolated from this standard curve.

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