Human GLA ELISA Kit

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  • Alternative name

    Human alpha-D-galactosidase A ELISA Kit;Human Alpha-D-galactoside galactohydrolase ELISA Kit;Human melibiase ELISA Kit;Human GALA ELISA Kit;Human galactosidase alpha ELISA Kit;Human alpha-galactosidase A ELISA Kit;Human agalsidase alfa ELISA Kit;Human alpha-D-galactoside galactohydrolase 1 ELISA Kit;Human alpha-gal A ELISA Kit;

  • Catalog
    E015935
  • species
    Human
  • GeneGLA
  • Standard CurveHuman GLA ELISA Kit
  • Other Species Mouse Gla ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Galactosidase Alpha (GLa). No significant cross-reactivity or interference between Galactosidase Alpha (GLa) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.55ng/mL.
  • Intended UseHuman GLA ELISA Kit allows for the in vitro quantitative determination of GLA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Alpha (GLa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Alpha (GLa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Alpha (GLa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactosidase Alpha (GLa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Alpha-galactosidase A Protein information
  • Uniprot ID AGAL_HUMAN
  • Uniprot AC P06280; Q6LER7;
  • UniGene Hs.69089;
  • GeneID 2717
  • KEGG hsa:2717;
  • Human Alpha-galactosidase A Protein SEQUENCE
  • SEQUENCE 429 AA; 48767 MW; 613F8BF21B107D7B CRC64;

    MQLRNPELHL GCALALRFLA LVSWDIPGAR ALDNGLARTP TMGWLHWERF

    MCNLDCQEEP DSCISEKLFM EMAELMVSEG WKDAGYEYLC IDDCWMAPQR

    DSEGRLQADP QRFPHGIRQL ANYVHSKGLK LGIYADVGNK TCAGFPGSFG

    YYDIDAQTFA DWGVDLLKFD GCYCDSLENL ADGYKHMSLA LNRTGRSIVY

    SCEWPLYMWP FQKPNYTEIR QYCNHWRNFA DIDDSWKSIK SILDWTSFNQ

    ERIVDVAGPG GWNDPDMLVI GNFGLSWNQQ VTQMALWAIM AAPLFMSNDL

    RHISPQAKAL LQDKDVIAIN QDPLGKQGYQ LRQGDNFEVW ERPLSGLAWA

    VAMINRQEIG GPRSYTIAVA SLGKGVACNP ACFITQLLPV KRKLGFYEWT

    SRLRSHINPT GTVLLQLENT MQMSLKDLL




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