Human GABPA ELISA Kit

Alternative name
Human GABP subunit alpha ELISA Kit;Human nuclear respiratory factor 2 subunit alpha ELISA Kit;Human transcription factor E4TF1-60 ELISA Kit;Human E4TF1A ELISA Kit;Human E4TF1-60 ELISA Kit;Human NFT2 ELISA Kit;Human NRF2 ELISA Kit;Human NRF2A ELISA Kit;Human RCH04A07 ELISA Kit;Human GA binding protein transcription factor alpha subunit ELISA Kit;Human GA-binding protein alpha chain ELISA Kit;Human GA binding protein transcription factor alpha subunit 60kDa ELISA Kit;Human human nuclear respiratory factor-2 subunit alpha ELISA Kit;Human nuclear respiratory factor 2 alpha subunit ELISA Kit;
Catalog
E015887
species
Human
GeneGABPA
Standard Curve
Other Species Mouse Gabpa ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of GA Binding Protein Transcription Factor Alpha (GABPa). No significant cross-reactivity or interference between GA Binding Protein Transcription Factor Alpha (GABPa) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL..
Intended UseHuman GABPA ELISA Kit allows for the in vitro quantitative determination of GABPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GA Binding Protein Transcription Factor Alpha (GABPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GA Binding Protein Transcription Factor Alpha (GABPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GA Binding Protein Transcription Factor Alpha (GABPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GA Binding Protein Transcription Factor Alpha (GABPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human GA-binding protein alpha chain Protein information
Uniprot ID GABPA_HUMAN
Uniprot AC Q06546; Q12939;
UniGene Hs.473470;
GeneID 2551
KEGG hsa:2551;
Human GA-binding protein alpha chain Protein SEQUENCE
SEQUENCE 454 AA; 51295 MW; 1AF2ABBBC79191DD CRC64;
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN
LKKLLEPRLQ CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS
YQGIEPKLNI LEIVKPADTV EVVIDPDAHH AESEAHLVEE AQVITLDGTK
HITTISDETS EQVTRWAAAL EGYRKEQERL GIPYDPIQWS TDQVLHWVVW
VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL WSHLELLRKY
VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL
NQPELVAQKW GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV
CDLKTLIGYS AAELNRLVTE CEQKKLAKMQ LHGIAQPVTA VALATASLQT
EKDN
UCSC uc002ylx.5; human.;

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