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Human CSF3 ELISA Kit

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  • Alternative name

    Human G-CSF ELISA Kit;Human pluripoietin ELISA Kit;Human C17orf33 ELISA Kit;Human GCSF ELISA Kit;Human CSF3OS ELISA Kit;Human colony stimulating factor 3 ELISA Kit;Human granulocyte colony-stimulating factor ELISA Kit;Human colony stimulating factor 3 (granulocyte) ELISA Kit;Human filgrastim ELISA Kit;Human granulocyte-colony stimulating factor ELISA Kit;Human lenograstim ELISA Kit;
  • Catalog
    E015862
  • species
    Human
  • GeneCSF3
  • Other Species Mouse Csf3 ELISA KitBovine CSF3 ELISA KitCanine CSF3 ELISA KitPorcine CSF3 ELISA KitSheep CSF3 ELISA Kit
  • SpecificityThe Human G-CSF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human G-CSF proteins within the range of 7.8 pg/ml - 500 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity4 pg/ml.
  • Intended UseHuman CSF3 ELISA Kit allows for the in vitro quantitative determination of CSF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Human G-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates, Urine. This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.
  • Human Granulocyte colony-stimulating factor Protein information
  • Uniprot ID CSF3_HUMAN
  • Uniprot AC P09919; A8MXR7;
  • UniGene Hs.2233;
  • GeneID 1440
  • KEGG hsa:1440;
  • Human Granulocyte colony-stimulating factor Protein SEQUENCE

  • SEQUENCE 207 AA; 22293 MW; 421F635ECC776996 CRC64;

    MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ

    VRKIQGDGAA LQEKLVSECA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ

    ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI

    WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV

    LRHLAQP

  • UCSC uc002htp.4; human. [P09919-1];


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