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Human GPBAR1 ELISA Kit

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  • Alternative name

    Human G-protein coupled receptor GPCR19 ELISA Kit;Human hGPCR19 ELISA Kit;Human membrane-type receptor for bile acids ELISA Kit;Human M-BAR ELISA Kit;Human hBG37 ELISA Kit;Human BG37 ELISA Kit;Human TGR5 ELISA Kit;Human GPCR19 ELISA Kit;Human GPR131 ELISA Kit;Human G protein-coupled bile acid receptor 1 ELISA Kit;Human G-protein coupled bile acid receptor 1 ELISA Kit;Human G-protein coupled bile acid receptor BG37 ELISA Kit;Human membrane bile acid receptor ELISA Kit;
  • Catalog
    E015798
  • species
    Human
  • GeneGPBAR1
  • Other Species Bovine GPBAR1 ELISA KitMouse Gpbar1 ELISA KitRabbit GPBAR1 ELISA KitRat Gpbar1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GPBAR1. No significant cross-reactivity or interference between Human GPBAR1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.039 ng/ml
  • Intended UseHuman GPBAR1 ELISA Kit allows for the in vitro quantitative determination of GPBAR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPBAR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPBAR1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GPBAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GPBAR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human G-protein coupled bile acid receptor 1 Protein information
  • Uniprot ID GPBAR_HUMAN
  • Uniprot AC Q8TDU6; B3KV35;
  • UniGene Hs.160954;
  • GeneID 151306
  • KEGG hsa:151306;
  • Human G-protein coupled bile acid receptor 1 Protein SEQUENCE

  • SEQUENCE 330 AA; 35248 MW; B3A1AEDADEAFE021 CRC64;

    MTPNSTGEVP SPIPKGALGL SLALASLIIT ANLLLALGIA WDRRLRSPPA

    GCFFLSLLLA GLLTGLALPT LPGLWNQSRR GYWSCLLVYL APNFSFLSLL

    ANLLLVHGER YMAVLRPLQP PGSIRLALLL TWAGPLLFAS LPALGWNHWT

    PGANCSSQAI FPAPYLYLEV YGLLLPAVGA AAFLSVRVLA TAHRQLQDIC

    RLERAVCRDE PSALARALTW RQARAQAGAM LLFGLCWGPY VATLLLSVLA

    YEQRPPLGPG TLLSLLSLGS ASAAAVPVAM GLGDQRYTAP WRAAAQRCLQ

    GLWGRASRDS PGPSIAYHPS SQSSVDLDLN

  • UCSC uc010zjw.1; human.;



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