Human GPRC5A ELISA Kit

Alternative name
Human G-protein coupled receptor family C group 5 member A ELISA Kit;Human phorbol ester induced gene 1 ELISA Kit;Human PEIG-1 ELISA Kit;Human retinoic acid-induced gene 1 protein ELISA Kit;Human RAIG-1 ELISA Kit;Human GPCR5A ELISA Kit;Human RAI3 ELISA Kit;Human RAIG1 ELISA Kit;Human TIG1 ELISA Kit;Human G protein-coupled receptor class C group 5 member A ELISA Kit;Human retinoic acid-induced protein 3 ELISA Kit;Human TPA induced gene 1 ELISA Kit;Human orphan G-protein-coupling receptor PEIG-1 ELISA Kit;Human phorbol ester induced protein-1 ELISA Kit;Human retinoic acid induced 3 ELISA Kit;Human retinoic acid responsive ELISA Kit;
Catalog
E015790
species
Human
GeneGPRC5A
Standard Curve
Other Species Mouse Gprc5a ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A). No significant cross-reactivity or interference between G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.117ng/mL.
Intended UseHuman GPRC5A ELISA Kit allows for the in vitro quantitative determination of GPRC5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Retinoic acid-induced protein 3 Protein information
Uniprot ID RAI3_HUMAN
Uniprot AC Q8NFJ5; B3KV45; O95357;
UniGene Hs.631733;
GeneID 9052
KEGG hsa:9052;
Human Retinoic acid-induced protein 3 Protein SEQUENCE
SEQUENCE 357 AA; 40251 MW; 7BEB524BF6F307E5 CRC64;
MATTVPDGCR NGLKSKYYRL CDKAEAWGIV LETVATAGVV TSVAFMLTLP
ILVCKVQDSN RRKMLPTQFL FLLGVLGIFG LTFAFIIGLD GSTGPTRFFL
FGILFSICFS CLLAHAVSLT KLVRGRKPLS LLVILGLAVG FSLVQDVIAI
EYIVLTMNRT NVNVFSELSA PRRNEDFVLL LTYVLFLMAL TFLMSSFTFC
GSFTGWKRHG AHIYLTMLLS IAIWVAWITL LMLPDFDRRW DDTILSSALA
ANGWVFLLAY VSPEFWLLTK QRNPMDYPVE DAFCKPQLVK KSYGVENRAY
SQEEITQGFE ETGDTLYAPY STHFQLQNQP PQKEFSIPRA HAWPSPYKDY
EVKKEGS
UCSC uc001rba.4; human.;

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Human GPRC5A ELISA Kit