Human GPR78 ELISA Kit

Alternative name
Human G protein-coupled receptor 78 ELISA Kit;Human G-protein coupled receptor 78 ELISA Kit;
Catalog
E015785
species
Human
GeneGPR78
Standard Curve
Other Species Mouse GPR78 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Coupled Receptor 78 (GPR78). No significant cross-reactivity or interference between G Protein Coupled Receptor 78 (GPR78) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.059ng/mL.
Intended UseHuman GPR78 ELISA Kit allows for the in vitro quantitative determination of GPR78 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 78 (GPR78). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 78 (GPR78). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 78 (GPR78), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor 78 (GPR78) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human G-protein coupled receptor 78 Protein information
Uniprot ID GPR78_HUMAN
Uniprot AC Q96P69; Q8NGV3;
UniGene Hs.740355;
GeneID 27201
KEGG hsa:27201;
Human G-protein coupled receptor 78 Protein SEQUENCE
SEQUENCE 363 AA; 39332 MW; 2C0052A0A1A22A93 CRC64;
MGPGEALLAG LLVMVLAVAL LSNALVLLCC AYSAELRTRA SGVLLVNLSL
GHLLLAALDM PFTLLGVMRG RTPSAPGACQ VIGFLDTFLA SNAALSVAAL
SADQWLAVGF PLRYAGRLRP RYAGLLLGCA WGQSLAFSGA ALGCSWLGYS
SAFASCSLRL PPEPERPRFA AFTATLHAVG FVLPLAVLCL TSLQVHRVAR
RHCQRMDTVT MKALALLADL HPSVRQRCLI QQKRRRHRAT RKIGIAIATF
LICFAPYVMT RLAELVPFVT VNAQWGILSK CLTYSKAVAD PFTYSLLRRP
FRQVLAGMVH RLLKRTPRPA STHDSSLDVA GMVHQLLKRT PRPASTHNGS
VDTENDSCLQ QTH
UCSC uc003glk.5; human.;

Category

Human GPR78 ELISA Kit

Human G protein-coupled receptor 78 ELISA Kit;Human G-protein coupled receptor 78 ELISA Kit;