SpecificityThis assay has high sensitivity and excellent specificity for detection of Human alphaZGP1. No significant cross-reactivity or interference between Human alphaZGP1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between Human alphaZGP1 and all the analogues, therefore, cross reaction may still exist.
Intended UseHuman alphaZGP1 ELISA Kit allows for the in vitro quantitative determination of alphaZGP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human alpha ZGP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human alphaZGP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human alphaZGP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human alphaZGP1 can be calculated.