Human GPR35 ELISA Kit

Alternative name
Human kynurenic acid receptor ELISA Kit;Human KYNA receptor ELISA Kit;Human G protein-coupled receptor 35 ELISA Kit;Human G-protein coupled receptor 35 ELISA Kit;
Catalog
E015776
species
Human
GeneGPR35
Standard Curve
Other Species Mouse Gpr35 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Coupled Receptor 35 (GPR35). No significant cross-reactivity or interference between G Protein Coupled Receptor 35 (GPR35) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman GPR35 ELISA Kit allows for the in vitro quantitative determination of GPR35 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 35 (GPR35). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 35 (GPR35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 35 (GPR35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor 35 (GPR35) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human G-protein coupled receptor 35 Protein information
Uniprot ID GPR35_HUMAN
Uniprot AC Q9HC97; J3KR30; O43495; Q17R58; Q4VBN5; Q4ZFV2; Q6FHI8; Q86UH4;
UniGene Hs.112218;
GeneID 2859
KEGG hsa:2859;
Human G-protein coupled receptor 35 Protein SEQUENCE
SEQUENCE 309 AA; 34072 MW; 97734FB7231B26F0 CRC64;
MNGTYNTCGS SDLTWPPAIK LGFYAYLGVL LVLGLLLNSL ALWVFCCRMQ
QWTETRIYMT NLAVADLCLL CTLPFVLHSL RDTSDTPLCQ LSQGIYLTNR
YMSISLVTAI AVDRYVAVRH PLRARGLRSP RQAAAVCAVL WVLVIGSLVA
RWLLGIQEGG FCFRSTRHNF NSMAFPLLGF YLPLAVVVFC SLKVVTALAQ
RPPTDVGQAE ATRKAARMVW ANLLVFVVCF LPLHVGLTVR LAVGWNACAL
LETIRRALYI TSKLSDANCC LDAICYYYMA KEFQEASALA VAPSAKAHKS
QDSLCVTLA
UCSC uc002vzs.4; human. [Q9HC97-1];

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Category

Human GPR35 ELISA Kit

Human kynurenic acid receptor ELISA Kit;Human KYNA receptor ELISA Kit;Human G protein-coupled receptor 35 ELISA Kit;Human G-protein coupled receptor 35 ELISA Kit;