SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Coupled Receptor 120 (GPR120).
No significant cross-reactivity or interference between G Protein Coupled Receptor 120 (GPR120) and analogues was observed.
Intended UseHuman GPR120 ELISA Kit allows for the in vitro quantitative determination of GPR120 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 120 (GPR120). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 120 (GPR120). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 120 (GPR120), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor 120 (GPR120) in the samples is then determined by comparing the O.D. of the samples to the standard curve.