Human GNAI3 ELISA Kit

Alternative name
Human g(i) alpha-3 ELISA Kit;Human 87U6 ELISA Kit;Human ARCND1 ELISA Kit;Human G protein subunit alpha i3 ELISA Kit;Human guanine nucleotide-binding protein G(k) subunit alpha ELISA Kit;Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 ELISA Kit;
Catalog
E015761
species
Human
GeneGNAI3
Standard Curve
Other Species Mouse Gnai3 ELISA Kit Guinea Pig GNAI3 ELISA Kit Hamster GNAI3 ELISA Kit Rat Gnai3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3). No significant cross-reactivity or interference between G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman GNAI3 ELISA Kit allows for the in vitro quantitative determination of GNAI3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Guanine nucleotide-binding protein G(k) subunit alpha Protein information
Uniprot ID GNAI3_HUMAN
Uniprot AC P08754; P17539; Q5TZX1;
UniGene Hs.73799; Hs.741171;
GeneID 2773
KEGG hsa:2773;
Human Guanine nucleotide-binding protein G(k) subunit alpha Protein SEQUENCE
SEQUENCE 354 AA; 40532 MW; EAB6B4DD3646BC01 CRC64;
MGCTLSAEDK AAVERSKMID RNLREDGEKA AKEVKLLLLG AGESGKSTIV
KQMKIIHEDG YSEDECKQYK VVVYSNTIQS IIAIIRAMGR LKIDFGEAAR
ADDARQLFVL AGSAEEGVMT PELAGVIKRL WRDGGVQACF SRSREYQLND
SASYYLNDLD RISQSNYIPT QQDVLRTRVK TTGIVETHFT FKDLYFKMFD
VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF
DSICNNKWFT ETSIILFLNK KDLFEEKIKR SPLTICYPEY TGSNTYEEAA
AYIQCQFEDL NRRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKE
CGLY
UCSC uc001dxz.4; human.;

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Human GNAI3 ELISA Kit

Human g(i) alpha-3 ELISA Kit;Human 87U6 ELISA Kit;Human ARCND1 ELISA Kit;Human G protein subunit alpha i3 ELISA Kit;Human guanine nucleotide-binding protein G(k) subunit alpha ELISA Kit;Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 ELISA Kit;