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Human FXYD3 ELISA Kit

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  • Alternative name

    Human chloride conductance inducer protein Mat-8 ELISA Kit;Human mammary tumor 8 kDa protein ELISA Kit;Human Phospholemman-like ELISA Kit;Human MAT8 ELISA Kit;Human PLML ELISA Kit;Human FXYD domain containing ion transport regulator 3 ELISA Kit;Human FXYD domain-containing ion transport regulator 3 ELISA Kit;Human phospholemman-like protein ELISA Kit;
  • Catalog
    E015703
  • species
    Human
  • GeneFXYD3
  • Standard CurveHuman FXYD3 ELISA Kit
  • Other Species Mouse Fxyd3 ELISA KitPorcine FXYD3 ELISA KitRat Fxyd3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FXYD3. No significant cross-reactivity or interference between FXYD3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FXYD3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman FXYD3 ELISA Kit allows for the in vitro quantitative determination of FXYD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This FXYD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of human FXYD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||FXYD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FXYD3 antibody and an FXYD3-HRP conjugate. The assay sample and buffer are incubated together with FXYD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FXYD3 concentration since FXYD3 from samples and FXYD3-HRP conjugate compete for the anti-FXYD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FXYD3 from the sample, fewer sites are left to bind FXYD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXYD3 concentration in each sample is interpolated from this standard curve.
  • Human FXYD domain-containing ion transport regulator 3 Protein information
  • Uniprot ID FXYD3_HUMAN
  • Uniprot AC Q14802; A6NDE0; C9JDU2; F5H174; F8WB34; Q13211; Q6IB59;
  • UniGene Hs.301350;
  • GeneID 5349
  • KEGG hsa:5349;
  • Human FXYD domain-containing ion transport regulator 3 Protein SEQUENCE

  • SEQUENCE 87 AA; 9263 MW; 6D674D668EB32493 CRC64;

    MQKVTLGLLV FLAGFPVLDA NDLEDKNSPF YYDWHSLQVG GLICAGVLCA

    MGIIIVMSAK CKCKFGQKSG HHPGETPPLI TPGSAQS

  • UCSC uc002nxv.4; human. [Q14802-1];


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