Human FH ELISA Kit

Alternative name
Human fumarase ELISA Kit;Human FMRD ELISA Kit;Human HLRCC ELISA Kit;Human LRCC ELISA Kit;Human MCL ELISA Kit;Human MCUL1 ELISA Kit;Human fumarate hydratase ELISA Kit;Human fumarate hydratase, mitochondrial ELISA Kit;
Catalog
E015678
species
Human
GeneFH
Other Species Mouse Fh ELISA Kit Monkey FH ELISA Kit Porcine FH ELISA Kit Rat Fh ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human FH. No significant cross-reactivity or interference between human FH and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human FH is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O
Intended UseHuman FH ELISA Kit allows for the in vitro quantitative determination of FH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Fumarate hydratase, mitochondrial Protein information
Uniprot ID FUMH_HUMAN
Uniprot AC P07954; B1ANK7;
UniGene Hs.592490;
GeneID 2271
KEGG hsa:2271;
Human Fumarate hydratase, mitochondrial Protein SEQUENCE
SEQUENCE 510 AA; 54637 MW; 86F91F95DC046F64 CRC64;
MYRALRLLAR SRPLVRAPAA ALASAPGLGG AAVPSFWPPN AARMASQNSF
RIEYDTFGEL KVPNDKYYGA QTVRSTMNFK IGGVTERMPT PVIKAFGILK
RAAAEVNQDY GLDPKIANAI MKAADEVAEG KLNDHFPLVV WQTGSGTQTN
MNVNEVISNR AIEMLGGELG SKIPVHPNDH VNKSQSSNDT FPTAMHIAAA
IEVHEVLLPG LQKLHDALDA KSKEFAQIIK IGRTHTQDAV PLTLGQEFSG
YVQQVKYAMT RIKAAMPRIY ELAAGGTAVG TGLNTRIGFA EKVAAKVAAL
TGLPFVTAPN KFEALAAHDA LVELSGAMNT TACSLMKIAN DIRFLGSGPR
SGLGELILPE NEPGSSIMPG KVNPTQCEAM TMVAAQVMGN HVAVTVGGSN
GHFELNVFKP MMIKNVLHSA RLLGDASVSF TENCVVGIQA NTERINKLMN
ESLMLVTALN PHIGYDKAAK IAKTAHKNGS TLKETAIELG YLTAEQFDEW
VKPKDMLGPK
UCSC uc001hyx.4; human. [P07954-1];

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Category

Human FH ELISA Kit

Human fumarase ELISA Kit;Human FMRD ELISA Kit;Human HLRCC ELISA Kit;Human LRCC ELISA Kit;Human MCL ELISA Kit;Human MCUL1 ELISA Kit;Human fumarate hydratase ELISA Kit;Human fumarate hydratase, mitochondrial ELISA Kit;