Human FZD4 ELISA Kit

Alternative name
Human Fz-4 ELISA Kit;Human hFz4 ELISA Kit;Human FzE4 ELISA Kit;Human CD344 ELISA Kit;Human EVR1 ELISA Kit;Human FEVR ELISA Kit;Human FZD4S ELISA Kit;Human Fz4 ELISA Kit;Human GPCR ELISA Kit;Human frizzled class receptor 4 ELISA Kit;Human frizzled-4 ELISA Kit;Human WNT receptor frizzled-4 ELISA Kit;Human frizzled 4, seven transmembrane spanning receptor ELISA Kit;Human frizzled family receptor 4 ELISA Kit;Human frizzled homolog 4 ELISA Kit;
Catalog
E015590
species
Human
GeneFZD4
Other Species Mouse Fzd4 ELISA Kit Chicken FZD4 ELISA Kit Rat Fzd4 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseHuman FZD4 ELISA Kit allows for the in vitro quantitative determination of FZD4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology anti-Human FZD4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FZD4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FZD4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FZD4 can be calculated.
Human Frizzled-4 Protein information
Uniprot ID FZD4_HUMAN
Uniprot AC Q9ULV1; A8K9Q3; Q14C97; Q6S9E4;
UniGene Hs.591968;
GeneID 8322
KEGG hsa:8322;
Human Frizzled-4 Protein SEQUENCE
SEQUENCE 537 AA; 59881 MW; E0A83ECEC560A381 CRC64;
MAWRGAGPSV PGAPGGVGLS LGLLLQLLLL LGPARGFGDE EERRCDPIRI
SMCQNLGYNV TKMPNLVGHE LQTDAELQLT TFTPLIQYGC SSQLQFFLCS
VYVPMCTEKI NIPIGPCGGM CLSVKRRCEP VLKEFGFAWP ESLNCSKFPP
QNDHNHMCME GPGDEEVPLP HKTPIQPGEE CHSVGTNSDQ YIWVKRSLNC
VLKCGYDAGL YSRSAKEFTD IWMAVWASLC FISTAFTVLT FLIDSSRFSY
PERPIIFLSM CYNIYSIAYI VRLTVGRERI SCDFEEAAEP VLIQEGLKNT
GCAIIFLLMY FFGMASSIWW VILTLTWFLA AGLKWGHEAI EMHSSYFHIA
AWAIPAVKTI VILIMRLVDA DELTGLCYVG NQNLDALTGF VVAPLFTYLV
IGTLFIAAGL VALFKIRSNL QKDGTKTDKL ERLMVKIGVF SVLYTVPATC
VIACYFYEIS NWALFRYSAD DSNMAVEMLK IFMSLLVGIT SGMWIWSAKT
LHTWQKCSNR LVNSGKVKRE KRGNGWVKPG KGSETVV
UCSC uc001pce.4; human.;

Category

Human FZD4 ELISA Kit