Human a1AT ELISA Kit

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  • Alternative name

    Alpha-1-antitrypsin ELISA KIT; Alpha-1 protease inhibitor ELISA KIT; Alpha-1-antiproteinase ELISA KIT; Serpin A1SERPINA1 ELISA KIT; AAT ELISA KIT; PI ELISA KIT; SPAAT ELISA KIT

  • Catalog
  • species
  • Genea1AT
  • Standard CurveHuman a1AT ELISA Kit
  • Other Species Mouse a1AT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT). No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.24ng/mL.
  • Intended UseHuman a1AT ELISA Kit allows for the in vitro quantitative determination of a1AT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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