SensitivityThe minimum detectable dose of human F-TESTO is typically less than 3.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human F-TESTO concentration that could be differentiated from zero. It was determine
Intended UseHuman F-TESTO ELISA Kit allows for the in vitro quantitative determination of F-TESTO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for testosterone and Horseradish Peroxidase (HRP) conjugated testosterone. The competitive inhibition reaction is launched between with HRP labeled testosterone and unlabeled testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of F-TESTO in the sample. The color development is stopped and the intensity of the color is measured.