Human FHIT ELISA Kit

Alternative name
Human AP3A hydrolase ELISA Kit;Human AP3Aase ELISA Kit;Human diadenosine 5',5'''-P1,P3-triphosphate hydrolase ELISA Kit;Human dinucleosidetriphosphatase ELISA Kit;Human Fragile histidine triad protein ELISA Kit;Human FRA3B ELISA Kit;Human fragile histidine triad ELISA Kit;Human bis(5'-adenosyl)-triphosphatase ELISA Kit;
Catalog
E015492
species
Human
GeneFHIT
SpecificityThis assay has high sensitivity and excellent specificity for detection of human FHIT. No significant cross-reactivity or interference between human FHIT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human FHIT is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman FHIT ELISA Kit allows for the in vitro quantitative determination of FHIT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FHIT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FHIT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FHIT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FHIT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Bis(5'-adenosyl)-triphosphatase Protein information
Uniprot ID FHIT_HUMAN
Uniprot AC P49789; A2IAS9; A2IAT0; A2IAT6; A8K1A9; Q45QG9; Q6IU12;
UniGene Hs.655995;
GeneID 2272
KEGG hsa:2272;
Human Bis(5'-adenosyl)-triphosphatase Protein SEQUENCE
SEQUENCE 147 AA; 16858 MW; 14D85961A19ECF3E CRC64;
MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL
RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL
PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQ
UCSC uc003dkx.5; human.;

Human FHIT ELISA Kit

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