Human FPGS ELISA Kit

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  • Alternative name

    Human folylpoly-gamma-glutamate synthetase ELISA Kit;Human FPGS ELISA Kit;Human tetrahydrofolylpolyglutamate synthase ELISA Kit;Human tetrahydrofolate synthase ELISA Kit;Human folylpolyglutamate synthase ELISA Kit;Human folylpolyglutamate synthase, mitochondrial ELISA Kit;

  • Catalog
    E015482
  • species
    Human
  • GeneFPGS
  • Standard CurveHuman FPGS ELISA Kit
  • Other Species Mouse Fpgs ELISA KitBovine FPGS ELISA KitHamster FPGS ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FPGS . No significant cross-reactivity or interference between FPGS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.375pg/ml
  • Intended UseHuman FPGS ELISA Kit allows for the in vitro quantitative determination of FPGS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FPGSantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FPGS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FPGS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FPGS can be calculated.
  • Human Folylpolyglutamate synthase, mitochondrial Protein information
  • Uniprot ID FOLC_HUMAN
  • Uniprot AC Q05932; B3KPW4; B7Z2Z3; F5H0K6; Q5JU19; Q5JU22; Q6P2P6;
  • UniGene Hs.335084;
  • GeneID 2356
  • KEGG hsa:2356;
  • Human Folylpolyglutamate synthase, mitochondrial Protein SEQUENCE
  • SEQUENCE 587 AA; 64609 MW; 5AF81409F5F77E5C CRC64;

    MSRARSHLRA ALFLAAASAR GITTQVAARR GLSAWPVPQE PSMEYQDAVR

    MLNTLQTNAG YLEQVKRQRG DPQTQLEAME LYLARSGLQV EDLDRLNIIH

    VTGTKGKGST CAFTECILRS YGLKTGFFSS PHLVQVRERI RINGQPISPE

    LFTKYFWRLY HRLEETKDGS CVSMPPYFRF LTLMAFHVFL QEKVDLAVVE

    VGIGGAYDCT NIIRKPVVCG VSSLGIDHTS LLGDTVEKIA WQKGGIFKQG

    VPAFTVLQPE GPLAVLRDRA QQISCPLYLC PMLEALEEGG PPLTLGLEGE

    HQRSNAALAL QLAHCWLQRQ DRHGAGEPKA SRPGLLWQLP LAPVFQPTSH

    MRLGLRNTEW PGRTQVLRRG PLTWYLDGAH TASSAQACVR WFRQALQGRE

    RPSGGPEVRV LLFNATGDRD PAALLKLLQP CQFDYAVFCP NLTEVSSTGN

    ADQQNFTVTL DQVLLRCLEH QQHWNHLDEE QASPDLWSAP SPEPGGSASL

    LLAPHPPHTC SASSLVFSCI SHALQWISQG RDPIFQPPSP PKGLLTHPVA

    HSGASILREA AAIHVLVTGS LHLVGGVLKL LEPALSQ

  • UCSC uc004bsg.3; human. [Q05932-1];




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