Human FOXO1 ELISA Kit

Alternative name
Human forkhead box protein O1A ELISA Kit;Human Forkhead in rhabdomyosarcoma ELISA Kit;Human FKHR ELISA Kit;Human FOXO1A ELISA Kit;Human FKH1 ELISA Kit;Human forkhead box O1 ELISA Kit;Human forkhead box protein O1 ELISA Kit;Human forkhead, Drosophila, homolog of, in rhabdomyosarcoma ELISA Kit;
Catalog
E015408
species
Human
GeneFOXO1
Standard Curve
Other Species Mouse Foxo1 ELISA Kit Bovine FOXO1 ELISA Kit Porcine FOXO1 ELISA Kit Rat Foxo1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of FOXO1. No significant cross-reactivity or interference between FOXO1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FOXO1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman FOXO1 ELISA Kit allows for the in vitro quantitative determination of FOXO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: FOXO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOXO1 antibody and an FOXO1-HRP conjugate. The assay sample and buffer are incubated together with FOXO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOXO1 concentration since FOXO1 from samples and FOXO1-HRP conjugate compete for the anti-FOXO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FOXO1 from the sample, fewer sites are left to bind FOXO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOXO1 concentration in each sample is interpolated from this standard curve.
Human Forkhead box protein O1 Protein information
Uniprot ID FOXO1_HUMAN
Uniprot AC Q12778; O43523; Q5VYC7; Q6NSK6;
UniGene Hs.370666;
GeneID 2308
KEGG hsa:2308;
Human Forkhead box protein O1 Protein SEQUENCE
SEQUENCE 655 AA; 69662 MW; 6DEF6C994BDFDBAB CRC64;
MAEAPQVVEI DPDFEPLPRP RSCTWPLPRP EFSQSNSATS SPAPSGSAAA
NPDAAAGLPS ASAAAVSADF MSNLSLLEES EDFPQAPGSV AAAVAAAAAA
AATGGLCGDF QGPEAGCLHP APPQPPPPGP LSQHPPVPPA AAGPLAGQPR
KSSSSRRNAW GNLSYADLIT KAIESSAEKR LTLSQIYEWM VKSVPYFKDK
GDSNSSAGWK NSIRHNLSLH SKFIRVQNEG TGKSSWWMLN PEGGKSGKSP
RRRAASMDNN SKFAKSRSRA AKKKASLQSG QEGAGDSPGS QFSKWPASPG
SHSNDDFDNW STFRPRTSSN ASTISGRLSP IMTEQDDLGE GDVHSMVYPP
SAAKMASTLP SLSEISNPEN MENLLDNLNL LSSPTSLTVS TQSSPGTMMQ
QTPCYSFAPP NTSLNSPSPN YQKYTYGQSS MSPLPQMPIQ TLQDNKSSYG
GMSQYNCAPG LLKELLTSDS PPHNDIMTPV DPGVAQPNSR VLGQNVMMGP
NSVMSTYGSQ ASHNKMMNPS SHTHPGHAQQ TSAVNGRPLP HTVSTMPHTS
GMNRLTQVKT PVQVPLPHPM QMSALGGYSS VSSCNGYGRM GLLHQEKLPS
DLDGMFIERL DCDMESIIRN DLMDGDTLDF NFDNVLPNQS FPHSVKTTTH
SWVSG
UCSC uc001uxl.5; human.;

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Category

Human FOXO1 ELISA Kit

Human forkhead box protein O1A ELISA Kit;Human Forkhead in rhabdomyosarcoma ELISA Kit;Human FKHR ELISA Kit;Human FOXO1A ELISA Kit;Human FKH1 ELISA Kit;Human forkhead box O1 ELISA Kit;Human forkhead box protein O1 ELISA Kit;Human forkhead, Drosophila, homolog of, in rhabdomyosarcoma ELISA Kit;