Human FOXO3 ELISA Kit

Alternative name
Human AF6q21 protein ELISA Kit;Human forkhead in rhabdomyosarcoma-like 1 ELISA Kit;Human AF6q21 ELISA Kit;Human FKHRL1 ELISA Kit;Human FKHRL1P2 ELISA Kit;Human FOXO2 ELISA Kit;Human FOXO3A ELISA Kit;Human forkhead box O3 ELISA Kit;Human forkhead box protein O3 ELISA Kit;Human forkhead box O3A ELISA Kit;Human forkhead homolog (rhabdomyosarcoma) like 1 ELISA Kit;Human forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 ELISA Kit;
Catalog
E015386
species
Human
GeneFOXO3
Other Species Mouse Foxo3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human FOXO3. No significant cross-reactivity or interference between Human FOXO3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.39 pg/ml
Intended UseHuman FOXO3 ELISA Kit allows for the in vitro quantitative determination of FOXO3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FOXO3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FOXO3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FOXO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FOXO3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Forkhead box protein O3 Protein information
Uniprot ID FOXO3_HUMAN
Uniprot AC O43524; B4DVZ6; E1P5E6; O15171; Q5T2I7; Q9BZ04;
UniGene Hs.220950;
GeneID 2309
KEGG hsa:2309;
Human Forkhead box protein O3 Protein SEQUENCE
SEQUENCE 673 AA; 71277 MW; E5B4E830665A9982 CRC64;
MAEAPASPAP LSPLEVELDP EFEPQSRPRS CTWPLQRPEL QASPAKPSGE
TAADSMIPEE EDDEDDEDGG GRAGSAMAIG GGGGSGTLGS GLLLEDSARV
LAPGGQDPGS GPATAAGGLS GGTQALLQPQ QPLPPPQPGA AGGSGQPRKC
SSRRNAWGNL SYADLITRAI ESSPDKRLTL SQIYEWMVRC VPYFKDKGDS
NSSAGWKNSI RHNLSLHSRF MRVQNEGTGK SSWWIINPDG GKSGKAPRRR
AVSMDNSNKY TKSRGRAAKK KAALQTAPES ADDSPSQLSK WPGSPTSRSS
DELDAWTDFR SRTNSNASTV SGRLSPIMAS TELDEVQDDD APLSPMLYSS
SASLSPSVSK PCTVELPRLT DMAGTMNLND GLTENLMDDL LDNITLPPSQ
PSPTGGLMQR SSSFPYTTKG SGLGSPTSSF NSTVFGPSSL NSLRQSPMQT
IQENKPATFS SMSHYGNQTL QDLLTSDSLS HSDVMMTQSD PLMSQASTAV
SAQNSRRNVM LRNDPMMSFA AQPNQGSLVN QNLLHHQHQT QGALGGSRAL
SNSVSNMGLS ESSSLGSAKH QQQSPVSQSM QTLSDSLSGS SLYSTSANLP
VMGHEKFPSD LDLDMFNGSL ECDMESIIRS ELMDADGLDF NFDSLISTQN
VVGLNVGNFT GAKQASSQSW VPG
UCSC uc003psk.3; human. [O43524-1];

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Human FOXO3 ELISA Kit