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Alternative name
Focal adhesion kinase 1 ELISA KIT; Focal adhesion kinase-related nonkinase ELISA KIT; FRNK ELISA KIT; Protein phosphatase 1 regulatory subunit 71 ELISA KIT; PPP1R71 ELISA KIT; Protein-tyrosine kinase 2 ELISA KIT; p125FAK ELISA KIT; pp125FAKPTK2 ELISA KIT; FAK ELISA KIT; FAK1 ELISA KIT; FADK 1 ELISA KIT; FRNK ELISA KIT; PPP1R71 ELISA KIT
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Catalog
E015275
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species
Human
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GeneFAK
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Standard Curve
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Other Species
Mouse FAK ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Focal Adhesion Kinase (FAK).
No significant cross-reactivity or interference between Focal Adhesion Kinase (FAK) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.53ng/mL.
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Intended UseHuman FAK ELISA Kit allows for the in vitro quantitative determination of FAK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Focal Adhesion Kinase (FAK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Focal Adhesion Kinase (FAK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Focal Adhesion Kinase (FAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Focal Adhesion Kinase (FAK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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