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Human FLT1 ELISA Kit

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  • Alternative name

    Human VEGFR-1 ELISA Kit;Human fms-like tyrosine kinase 1 ELISA Kit;Human FLT-1 ELISA Kit;Human tyrosine-protein kinase FRT ELISA Kit;Human tyrosine-protein kinase receptor FLT ELISA Kit;Human FLT ELISA Kit;Human vascular permeability factor receptor ELISA Kit;Human FRT ELISA Kit;Human VEGFR1 ELISA Kit;Human fms related tyrosine kinase 1 ELISA Kit;Human vascular endothelial growth factor receptor 1 ELISA Kit;Human fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) ELISA Kit;
  • Catalog
    E015264
  • species
    Human
  • GeneFLT1
  • Other Species Mouse Flt1 ELISA KitMouse FLT1/VEGFR1 ELISA KitMouse VEGFR-1/Flt1 ELISA KitMouse VEGFR-2/Flt1 ELISA KitChicken FLT1 ELISA KitRat Flt1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human FLT1. No significant cross-reactivity or interference between Human FLT1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.039 ng/ml
  • Intended UseHuman FLT1 ELISA Kit allows for the in vitro quantitative determination of FLT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: VEGF receptors are receptors for vascular endothelial growth factor (VEGF). VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sVEGFR-1/sFlt1) which binds VEGF with high affinity. sVEGFR-1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sVEGFR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for sVEGFR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5 tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sVEGFR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sVEGFR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Vascular endothelial growth factor receptor 1 Protein information
  • Uniprot ID VGFR1_HUMAN
  • Uniprot AC B3FR89; B5A923; F5H5L6; O60722; P16057; Q12954;
  • UniGene Hs.594454;
  • GeneID 2321
  • KEGG hsa:2321;
  • Human Vascular endothelial growth factor receptor 1 Protein SEQUENCE

  • SEQUENCE 1338 AA; 150769 MW; FF3381EEFAF0787C CRC64;

    MVSYWDTGVL LCALLSCLLL TGSSSGSKLK DPELSLKGTQ HIMQAGQTLH

    LQCRGEAAHK WSLPEMVSKE SERLSITKSA CGRNGKQFCS TLTLNTAQAN

    HTGFYSCKYL AVPTSKKKET ESAIYIFISD TGRPFVEMYS EIPEIIHMTE

    GRELVIPCRV TSPNITVTLK KFPLDTLIPD GKRIIWDSRK GFIISNATYK

    EIGLLTCEAT VNGHLYKTNY LTHRQTNTII DVQISTPRPV KLLRGHTLVL

    NCTATTPLNT RVQMTWSYPD EKNKRASVRR RIDQSNSHAN IFYSVLTIDK

    MQNKDKGLYT CRVRSGPSFK SVNTSVHIYD KAFITVKHRK QQVLETVAGK

    RSYRLSMKVK AFPSPEVVWL KDGLPATEKS ARYLTRGYSL IIKDVTEEDA

    GNYTILLSIK QSNVFKNLTA TLIVNVKPQI YEKAVSSFPD PALYPLGSRQ

    ILTCTAYGIP QPTIKWFWHP CNHNHSEARC DFCSNNEESF ILDADSNMGN

    RIESITQRMA IIEGKNKMAS TLVVADSRIS GIYICIASNK VGTVGRNISF

    YITDVPNGFH VNLEKMPTEG EDLKLSCTVN KFLYRDVTWI LLRTVNNRTM

    HYSISKQKMA ITKEHSITLN LTIMNVSLQD SGTYACRARN VYTGEEILQK

    KEITIRDQEA PYLLRNLSDH TVAISSSTTL DCHANGVPEP QITWFKNNHK

    IQQEPGIILG PGSSTLFIER VTEEDEGVYH CKATNQKGSV ESSAYLTVQG

    TSDKSNLELI TLTCTCVAAT LFWLLLTLFI RKMKRSSSEI KTDYLSIIMD

    PDEVPLDEQC ERLPYDASKW EFARERLKLG KSLGRGAFGK VVQASAFGIK

    KSPTCRTVAV KMLKEGATAS EYKALMTELK ILTHIGHHLN VVNLLGACTK

    QGGPLMVIVE YCKYGNLSNY LKSKRDLFFL NKDAALHMEP KKEKMEPGLE

    QGKKPRLDSV TSSESFASSG FQEDKSLSDV EEEEDSDGFY KEPITMEDLI

    SYSFQVARGM EFLSSRKCIH RDLAARNILL SENNVVKICD FGLARDIYKN

    PDYVRKGDTR LPLKWMAPES IFDKIYSTKS DVWSYGVLLW EIFSLGGSPY

    PGVQMDEDFC SRLREGMRMR APEYSTPEIY QIMLDCWHRD PKERPRFAEL

    VEKLGDLLQA NVQQDGKDYI PINAILTGNS GFTYSTPAFS EDFFKESISA

    PKFNSGSSDD VRYVNAFKFM SLERIKTFEE LLPNATSMFD DYQGDSSTLL

    ASPMLKRFTW TDSKPKASLK IDLRVTSKSK ESGLSDVSRP SFCHSSCGHV

    SEGKRRFTYD HAELERKIAC CSPPPDYNSV VLYSTPPI

  • UCSC uc001usb.4; human. [P17948-1];


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