Human FLT3LG ELISA Kit

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  • Alternative name

    Human flt3 ligand ELISA Kit;Human FLT3L ELISA Kit;Human SL cytokine ELISA Kit;Human FL ELISA Kit;Human fms related tyrosine kinase 3 ligand ELISA Kit;Human fms-related tyrosine kinase 3 ligand ELISA Kit;

  • Catalog
    E015243
  • species
    Human
  • GeneFLT3LG
  • Other Species Mouse Flt3lg ELISA Kit
  • SpecificityNatural and recombinant human Flt-3ligand
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman FLT3LG ELISA Kit allows for the in vitro quantitative determination of FLT3LG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Flt-3ligand has been precoated onto 96-well plates. Standards(sf21, T27-P185) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Flt-3ligand amount of sample captured in plate. Background: Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
  • Human Fms-related tyrosine kinase 3 ligand Protein information
  • Uniprot ID FLT3L_HUMAN
  • Uniprot AC P49771; A0AVC2; B9EGH2; Q05C96;
  • UniGene Hs.428;
  • GeneID 2323
  • KEGG hsa:2323;
  • Human Fms-related tyrosine kinase 3 ligand Protein SEQUENCE
  • SEQUENCE 235 AA; 26416 MW; 73B95BF693B4CECF CRC64;

    MTVLAPAWSP TTYLLLLLLL SSGLSGTQDC SFQHSPISSD FAVKIRELSD

    YLLQDYPVTV ASNLQDEELC GGLWRLVLAQ RWMERLKTVA GSKMQGLLER

    VNTEIHFVTK CAFQPPPSCL RFVQTNISRL LQETSEQLVA LKPWITRQNF

    SRCLELQCQP DSSTLPPPWS PRPLEATAPT APQPPLLLLL LLPVGLLLLA

    AAWCLHWQRT RRRTPRPGEQ VPPVPSPQDL LLVEH

  • UCSC uc002pnu.4; human. [P49771-1];



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