Human FLII ELISA Kit

Alternative name
Human FLIL ELISA Kit;Human FLI ELISA Kit;Human Fli1 ELISA Kit;Human FLII, actin remodeling protein ELISA Kit;Human protein flightless-1 homolog ELISA Kit;Human flightless I actin binding protein ELISA Kit;Human flightless I homolog ELISA Kit;
Catalog
E015228
species
Human
GeneFLII
SpecificityThis assay has high sensitivity and excellent specificity for detection of Flightless I Homolog (FLII). No significant cross-reactivity or interference between Flightless I Homolog (FLII) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 29.2pg/mL.
Intended UseHuman FLII ELISA Kit allows for the in vitro quantitative determination of FLII , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flightless I Homolog (FLII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flightless I Homolog (FLII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flightless I Homolog (FLII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flightless I Homolog (FLII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein flightless-1 homolog Protein information
Uniprot ID FLII_HUMAN
Uniprot AC Q13045; B4DIL0; F5H407; J3QLG3;
UniGene Hs.513984;
GeneID 2314
KEGG hsa:2314;
Human Protein flightless-1 homolog Protein SEQUENCE
SEQUENCE 1269 AA; 144751 MW; 29AC7C07738B7B47 CRC64;
MEATGVLPFV RGVDLSGNDF KGGYFPENVK AMTSLRWLKL NRTGLCYLPE
ELAALQKLEH LSVSHNNLTT LHGELSSLPS LRAIVARANS LKNSGVPDDI
FKLDDLSVLD LSHNQLTECP RELENAKNML VLNLSHNSID TIPNQLFINL
TDLLYLDLSE NRLESLPPQM RRLVHLQTLV LNGNPLLHAQ LRQLPAMTAL
QTLHLRSTQR TQSNLPTSLE GLSNLADVDL SCNDLTRVPE CLYTLPSLRR
LNLSSNQITE LSLCIDQWVH VETLNLSRNQ LTSLPSAICK LSKLKKLYLN
SNKLDFDGLP SGIGKLTNLE EFMAANNNLE LVPESLCRCP KLRKLVLNKN
HLVTLPEAIH FLTEIEVLDV RENPNLVMPP KPADRAAEWY NIDFSLQNQL
RLAGASPATV AAAAAAGSGP KDPMARKMRL RRRKDSAQDD QAKQVLKGMS
DVAQEKNKKQ EESADARAPS GKVRRWDQGL EKPRLDYSEF FTEDVGQLPG
LTIWQIENFV PVLVEEAFHG KFYEADCYIV LKTFLDDSGS LNWEIYYWIG
GEATLDKKAC SAIHAVNLRN YLGAECRTVR EEMGDESEEF LQVFDNDISY
IEGGTASGFY TVEDTHYVTR MYRVYGKKNI KLEPVPLKGT SLDPRFVFLL
DRGLDIYVWR GAQATLSSTT KARLFAEKIN KNERKGKAEI TLLVQGQELP
EFWEALGGEP SEIKKHVPED FWPPQPKLYK VGLGLGYLEL PQINYKLSVE
HKQRPKVELM PRMRLLQSLL DTRCVYILDC WSDVFIWLGR KSPRLVRAAA
LKLGQELCGM LHRPRHATVS RSLEGTEAQV FKAKFKNWDD VLTVDYTRNA
EAVLQSPGLS GKVKRDAEKK DQMKADLTAL FLPRQPPMSL AEAEQLMEEW
NEDLDGMEGF VLEGKKFARL PEEEFGHFYT QDCYVFLCRY WVPVEYEEEE
KKEDKEEKAE GKEGEEATAE AEEKQPEEDF QCIVYFWQGR EASNMGWLTF
TFSLQKKFES LFPGKLEVVR MTQQQENPKF LSHFKRKFII HRGKRKAVQG
AQQPSLYQIR TNGSALCTRC IQINTDSSLL NSEFCFILKV PFESEDNQGI
VYAWVGRASD PDEAKLAEDI LNTMFDTSYS KQVINEGEEP ENFFWVGIGA
QKPYDDDAEY MKHTRLFRCS NEKGYFAVTE KCSDFCQDDL ADDDIMLLDN
GQEVYMWVGT QTSQVEIKLS LKACQVYIQH MRSKEHERPR RLRLVRKGNE
QHAFTRCFHA WSAFCKALA
UCSC uc002gsr.3; human. [Q13045-1];

Category

Human FLII ELISA Kit

Human FLIL ELISA Kit;Human FLI ELISA Kit;Human Fli1 ELISA Kit;Human FLII, actin remodeling protein ELISA Kit;Human protein flightless-1 homolog ELISA Kit;Human flightless I actin binding protein ELISA Kit;Human flightless I homolog ELISA Kit;