Human FLII ELISA Kit

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  • Alternative name

    Human FLIL ELISA Kit;Human FLI ELISA Kit;Human Fli1 ELISA Kit;Human FLII, actin remodeling protein ELISA Kit;Human protein flightless-1 homolog ELISA Kit;Human flightless I actin binding protein ELISA Kit;Human flightless I homolog ELISA Kit;

  • Catalog
    E015228
  • species
    Human
  • GeneFLII
  • Standard CurveHuman FLII ELISA Kit
  • Other Species Mouse Flii ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Flightless I Homolog (FLII). No significant cross-reactivity or interference between Flightless I Homolog (FLII) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 29.2pg/mL.
  • Intended UseHuman FLII ELISA Kit allows for the in vitro quantitative determination of FLII , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flightless I Homolog (FLII). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flightless I Homolog (FLII). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flightless I Homolog (FLII), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flightless I Homolog (FLII) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein flightless-1 homolog Protein information
  • Uniprot ID FLII_HUMAN
  • Uniprot AC Q13045; B4DIL0; F5H407; J3QLG3;
  • UniGene Hs.513984;
  • GeneID 2314
  • KEGG hsa:2314;
  • Human Protein flightless-1 homolog Protein SEQUENCE
  • SEQUENCE 1269 AA; 144751 MW; 29AC7C07738B7B47 CRC64;

    MEATGVLPFV RGVDLSGNDF KGGYFPENVK AMTSLRWLKL NRTGLCYLPE

    ELAALQKLEH LSVSHNNLTT LHGELSSLPS LRAIVARANS LKNSGVPDDI

    FKLDDLSVLD LSHNQLTECP RELENAKNML VLNLSHNSID TIPNQLFINL

    TDLLYLDLSE NRLESLPPQM RRLVHLQTLV LNGNPLLHAQ LRQLPAMTAL

    QTLHLRSTQR TQSNLPTSLE GLSNLADVDL SCNDLTRVPE CLYTLPSLRR

    LNLSSNQITE LSLCIDQWVH VETLNLSRNQ LTSLPSAICK LSKLKKLYLN

    SNKLDFDGLP SGIGKLTNLE EFMAANNNLE LVPESLCRCP KLRKLVLNKN

    HLVTLPEAIH FLTEIEVLDV RENPNLVMPP KPADRAAEWY NIDFSLQNQL

    RLAGASPATV AAAAAAGSGP KDPMARKMRL RRRKDSAQDD QAKQVLKGMS

    DVAQEKNKKQ EESADARAPS GKVRRWDQGL EKPRLDYSEF FTEDVGQLPG

    LTIWQIENFV PVLVEEAFHG KFYEADCYIV LKTFLDDSGS LNWEIYYWIG

    GEATLDKKAC SAIHAVNLRN YLGAECRTVR EEMGDESEEF LQVFDNDISY

    IEGGTASGFY TVEDTHYVTR MYRVYGKKNI KLEPVPLKGT SLDPRFVFLL

    DRGLDIYVWR GAQATLSSTT KARLFAEKIN KNERKGKAEI TLLVQGQELP

    EFWEALGGEP SEIKKHVPED FWPPQPKLYK VGLGLGYLEL PQINYKLSVE

    HKQRPKVELM PRMRLLQSLL DTRCVYILDC WSDVFIWLGR KSPRLVRAAA

    LKLGQELCGM LHRPRHATVS RSLEGTEAQV FKAKFKNWDD VLTVDYTRNA

    EAVLQSPGLS GKVKRDAEKK DQMKADLTAL FLPRQPPMSL AEAEQLMEEW

    NEDLDGMEGF VLEGKKFARL PEEEFGHFYT QDCYVFLCRY WVPVEYEEEE

    KKEDKEEKAE GKEGEEATAE AEEKQPEEDF QCIVYFWQGR EASNMGWLTF

    TFSLQKKFES LFPGKLEVVR MTQQQENPKF LSHFKRKFII HRGKRKAVQG

    AQQPSLYQIR TNGSALCTRC IQINTDSSLL NSEFCFILKV PFESEDNQGI

    VYAWVGRASD PDEAKLAEDI LNTMFDTSYS KQVINEGEEP ENFFWVGIGA

    QKPYDDDAEY MKHTRLFRCS NEKGYFAVTE KCSDFCQDDL ADDDIMLLDN

    GQEVYMWVGT QTSQVEIKLS LKACQVYIQH MRSKEHERPR RLRLVRKGNE

    QHAFTRCFHA WSAFCKALA

  • UCSC uc002gsr.3; human. [Q13045-1];



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