Human FLG ELISA Kit

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  • Alternative name

    Human ATOD2 ELISA Kit;Human filaggrin ELISA Kit;Human epidermal filaggrin ELISA Kit;

  • Catalog
    E015151
  • species
    Human
  • GeneFLG
  • Standard CurveHuman FLG ELISA Kit
  • Other Species Mouse Flg ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Filaggrin (FLG). No significant cross-reactivity or interference between Filaggrin (FLG) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.129ng/mL.
  • Intended UseHuman FLG ELISA Kit allows for the in vitro quantitative determination of FLG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filaggrin (FLG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filaggrin (FLG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filaggrin (FLG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filaggrin (FLG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Filaggrin Protein information
  • Uniprot ID FILA_HUMAN
  • Uniprot AC P20930; Q01720; Q5T583; Q9UC71;
  • UniGene Hs.654510;
  • GeneID 2312
  • KEGG hsa:2312;
  • Human Filaggrin Protein SEQUENCE
  • SEQUENCE 4061 AA; 435170 MW; 3F4B1181F04AD9C0 CRC64;

    MSTLLENIFA IINLFKQYSK KDKNTDTLSK KELKELLEKE FRQILKNPDD

    PDMVDVFMDH LDIDHNKKID FTEFLLMVFK LAQAYYESTR KENLPISGHK

    HRKHSHHDKH EDNKQEENKE NRKRPSSLER RNNRKGNKGR SKSPRETGGK

    RHESSSEKKE RKGYSPTHRE EEYGKNHHNS SKKEKNKTEN TRLGDNRKRL

    SERLEEKEDN EEGVYDYENT GRMTQKWIQS GHIATYYTIQ DEAYDTTDSL

    LEENKIYERS RSSDGKSSSQ VNRSRHENTS QVPLQESRTR KRRGSRVSQD

    RDSEGHSEDS ERHSGSASRN HHGSAWEQSR DGSRHPRSHD EDRASHGHSA

    DSSRQSGTRH AETSSRGQTA SSHEQARSSP GERHGSGHQQ SADSSRHSAT

    GRGQASSAVS DRGHRGSSGS QASDSEGHSE NSDTQSVSGH GKAGLRQQSH

    QESTRGRSGE RSGRSGSSLY QVSTHEQPDS AHGRTGTSTG GRQGSHHEQA

    RDSSRHSASQ EGQDTIRGHP GSSRGGRQGS HHEQSVNRSG HSGSHHSHTT

    SQGRSDASHG QSGSRSASRQ TRNEEQSGDG TRHSGSRHHE ASSQADSSRH

    SQVGQGQSSG PRTSRNQGSS VSQDSDSQGH SEDSERWSGS ASRNHHGSAQ

    EQSRDGSRHP RSHHEDRAGH GHSADSSRKS GTRHTQNSSS GQAASSHEQA

    RSSAGERHGS RHQLQSADSS RHSGTGHGQA SSAVRDSGHR GSSGSQATDS

    EGHSEDSDTQ SVSGHGQAGH HQQSHQESAR DRSGERSRRS GSFLYQVSTH

    KQSESSHGWT GPSTGVRQGS HHEQARDNSR HSASQDGQDT IRGHPGSSRR

    GRQGSHHEQS VDRSGHSGSH HSHTTSQGRS DASRGQSGSR SASRTTRNEE

    QSRDGSRHSG SRHHEASSHA DISRHSQAGQ GQSEGSRTSR RQGSSVSQDS

    DSEGHSEDSE RWSGSASRNH RGSAQEQSRH GSRHPRSHHE DRAGHGHSAD

    SSRQSGTPHA ETSSGGQAAS SHEQARSSPG ERHGSRHQQS ADSSRHSGIP

    RRQASSAVRD SGHWGSSGSQ ASDSEGHSEE SDTQSVSGHG QDGPHQQSHQ

    ESARDWSGGR SGRSGSFIYQ VSTHEQSESA HGRTRTSTGR RQGSHHEQAR

    DSSRHSASQE GQDTIRAHPG SRRGGRQGSH HEQSVDRSGH SGSHHSHTTS

    QGRSDASHGQ SGSRSASRQT RKDKQSGDGS RHSGSRHHEA ASWADSSRHS

    QVGQEQSSGS RTSRHQGSSV SQDSDSERHS DDSERLSGSA SRNHHGSSRE

    QSRDGSRHPG FHQEDRASHG HSADSSRQSG THHTESSSHG QAVSSHEQAR

    SSPGERHGSR HQQSADSSRH SGIGHRQASS AVRDSGHRGS SGSQVTNSEG

    HSEDSDTQSV SAHGQAGPHQ QSHKESARGQ SGESSGRSRS FLYQVSSHEQ

    SESTHGQTAP STGGRQGSRH EQARNSSRHS ASQDGQDTIR GHPGSSRGGR

    QGSYHEQSVD RSGHSGYHHS HTTPQGRSDA SHGQSGPRSA SRQTRNEEQS

    GDGSRHSGSR HHEPSTRAGS SRHSQVGQGE SAGSKTSRRQ GSSVSQDRDS

    EGHSEDSERR SESASRNHYG SAREQSRHGS RNPRSHQEDR ASHGHSAESS

    RQSGTRHAET SSGGQAASSQ EQARSSPGER HGSRHQQSAD SSTDSGTGRR

    QDSSVVGDSG NRGSSGSQAS DSEGHSEESD TQSVSAHGQA GPHQQSHQES

    TRGQSGERSG RSGSFLYQVS THEQSESAHG RTGPSTGGRQ RSRHEQARDS

    SRHSASQEGQ DTIRGHPGSS RGGRQGSHYE QSVDSSGHSG SHHSHTTSQE

    RSDVSRGQSG SRSVSRQTRN EKQSGDGSRH SGSRHHEASS RADSSRHSQV

    GQGQSSGPRT SRNQGSSVSQ DSDSQGHSED SERWSGSASR NHLGSAWEQS

    RDGSRHPGSH HEDRAGHGHS ADSSRQSGTR HTESSSRGQA ASSHEQARSS

    AGERHGSHHQ LQSADSSRHS GIGHGQASSA VRDSGHRGYS GSQASDSEGH

    SEDSDTQSVS AQGKAGPHQQ SHKESARGQS GESSGRSGSF LYQVSTHEQS

    ESTHGQSAPS TGGRQGSHYD QAQDSSRHSA SQEGQDTIRG HPGPSRGGRQ

    GSHQEQSVDR SGHSGSHHSH TTSQGRSDAS RGQSGSRSAS RKTYDKEQSG

    DGSRHSGSHH HEASSWADSS RHSLVGQGQS SGPRTSRPRG SSVSQDSDSE

    GHSEDSERRS GSASRNHHGS AQEQSRDGSR HPRSHHEDRA GHGHSAESSR

    QSGTHHAENS SGGQAASSHE QARSSAGERH GSHHQQSADS SRHSGIGHGQ

    ASSAVRDSGH RGSSGSQASD SEGHSEDSDT QSVSAHGQAG PHQQSHQEST

    RGRSAGRSGR SGSFLYQVST HEQSESAHGR TGTSTGGRQG SHHKQARDSS

    RHSTSQEGQD TIHGHPGSSS GGRQGSHYEQ LVDRSGHSGS HHSHTTSQGR

    SDASHGHSGS RSASRQTRND EQSGDGSRHS GSRHHEASSR ADSSGHSQVG

    QGQSEGPRTS RNWGSSFSQD SDSQGHSEDS ERWSGSASRN HHGSAQEQLR

    DGSRHPRSHQ EDRAGHGHSA DSSRQSGTRH TQTSSGGQAA SSHEQARSSA

    GERHGSHHQQ SADSSRHSGI GHGQASSAVR DSGHRGYSGS QASDNEGHSE

    DSDTQSVSAH GQAGSHQQSH QESARGRSGE TSGHSGSFLY QVSTHEQSES

    SHGWTGPSTR GRQGSRHEQA QDSSRHSASQ DGQDTIRGHP GSSRGGRQGY

    HHEHSVDSSG HSGSHHSHTT SQGRSDASRG QSGSRSASRT TRNEEQSGDG

    SRHSGSRHHE ASTHADISRH SQAVQGQSEG SRRSRRQGSS VSQDSDSEGH

    SEDSERWSGS ASRNHHGSAQ EQLRDGSRHP RSHQEDRAGH GHSADSSRQS

    GTRHTQTSSG GQAASSHEQA RSSAGERHGS HHQQSADSSR HSGIGHGQAS

    SAVRDSGHRG YSGSQASDNE GHSEDSDTQS VSAHGQAGSH QQSHQESARG

    RSGETSGHSG SFLYQVSTHE QSESSHGWTG PSTRGRQGSR HEQAQDSSRH

    SASQYGQDTI RGHPGSSRGG RQGYHHEHSV DSSGHSGSHH SHTTSQGRSD

    ASRGQSGSRS ASRTTRNEEQ SGDSSRHSVS RHHEASTHAD ISRHSQAVQG

    QSEGSRRSRR QGSSVSQDSD SEGHSEDSER WSGSASRNHR GSVQEQSRHG

    SRHPRSHHED RAGHGHSADR SRQSGTRHAE TSSGGQAASS HEQARSSPGE

    RHGSRHQQSA DSSRHSGIPR GQASSAVRDS RHWGSSGSQA SDSEGHSEES

    DTQSVSGHGQ AGPHQQSHQE SARDRSGGRS GRSGSFLYQV STHEQSESAH

    GRTRTSTGRR QGSHHEQARD SSRHSASQEG QDTIRGHPGS SRRGRQGSHY

    EQSVDRSGHS GSHHSHTTSQ GRSDASRGQS GSRSASRQTR NDEQSGDGSR

    HSWSHHHEAS TQADSSRHSQ SGQGQSAGPR TSRNQGSSVS QDSDSQGHSE

    DSERWSGSAS RNHRGSAQEQ SRDGSRHPTS HHEDRAGHGH SAESSRQSGT

    HHAENSSGGQ AASSHEQARS SAGERHGSHH QQSADSSRHS GIGHGQASSA

    VRDSGHRGSS GSQASDSEGH SEDSDTQSVS AHGQAGPHQQ SHQESTRGRS

    AGRSGRSGSF LYQVSTHEQS ESAHGRAGPS TGGRQGSRHE QARDSSRHSA

    SQEGQDTIRG HPGSRRGGRQ GSYHEQSVDR SGHSGSHHSH TTSQGRSDAS

    HGQSGSRSAS RETRNEEQSG DGSRHSGSRH HEASTQADSS RHSQSGQGES

    AGSRRSRRQG SSVSQDSDSE AYPEDSERRS ESASRNHHGS SREQSRDGSR

    HPGSSHRDTA SHVQSSPVQS DSSTAKEHGH FSSLSQDSAY HSGIQSRGSP

    HSSSSYHYQS EGTERQKGQS GLVWRHGSYG SADYDYGESG FRHSQHGSVS

    YNSNPVVFKE RSDICKASAF GKDHPRYYAT YINKDPGLCG HSSDISKQLG

    FSQSQRYYYY E

  • UCSC uc001ezu.1; human.;


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