Human FBLN5 ELISA Kit

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  • Alternative name

    Human FIBL-5 ELISA Kit;Human developmental arteries and neural crest EGF-like protein ELISA Kit;Human DANCE ELISA Kit;Human urine p50 protein ELISA Kit;Human UP50 ELISA Kit;Human ADCL2 ELISA Kit;Human ARCL1A ELISA Kit;Human ARMD3 ELISA Kit;Human EVEC ELISA Kit;Human HNARMD ELISA Kit;Human fibulin 5 ELISA Kit;Human fibulin-5 ELISA Kit;Human testis tissue sperm-binding protein Li 75n ELISA Kit;

  • Catalog
    E015105
  • species
    Human
  • GeneFBLN5
  • Standard CurveHuman FBLN5 ELISA Kit
  • Other Species Mouse Fbln5 ELISA KitBovine FBLN5 ELISA KitRat Fbln5 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman FBLN5 ELISA Kit allows for the in vitro quantitative determination of FBLN5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This FBLN5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FBLN5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY FBLN5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FBLN5. Standards or samples are then added to the microtiter plate wells and FBLN5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FBLN5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FBLN5 a readded to each well to ?sandwich? the FBLN5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FBLN5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBLN5 concentration in each sample is interpolated from this standard curve.
  • Human Fibulin-5 Protein information
  • Uniprot ID FBLN5_HUMAN
  • Uniprot AC Q9UBX5; O75966; Q6IAL4; Q6UWA3;
  • UniGene Hs.332708;
  • GeneID 10516
  • KEGG hsa:10516;
  • Human Fibulin-5 Protein SEQUENCE
  • SEQUENCE 448 AA; 50180 MW; 19FCA51FDA328003 CRC64;

    MPGIKRILTV TILALCLPSP GNAQAQCTNG FDLDRQSGQC LDIDECRTIP

    EACRGDMMCV NQNGGYLCIP RTNPVYRGPY SNPYSTPYSG PYPAAAPPLS

    APNYPTISRP LICRFGYQMD ESNQCVDVDE CATDSHQCNP TQICINTEGG

    YTCSCTDGYW LLEGQCLDID ECRYGYCQQL CANVPGSYSC TCNPGFTLNE

    DGRSCQDVNE CATENPCVQT CVNTYGSFIC RCDPGYELEE DGVHCSDMDE

    CSFSEFLCQH ECVNQPGTYF CSCPPGYILL DDNRSCQDIN ECEHRNHTCN

    LQQTCYNLQG GFKCIDPIRC EEPYLRISDN RCMCPAENPG CRDQPFTILY

    RDMDVVSGRS VPADIFQMQA TTRYPGAYYI FQIKSGNEGR EFYMRQTGPI

    SATLVMTRPI KGPREIQLDL EMITVNTVIN FRGSSVIRLR IYVSQYPF

  • UCSC uc001xzx.5; human.;


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