Human AATF ELISA Kit

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  • Alternative name

    Human Apoptosis-antagonizing transcription factor ELISA Kit;Human rb-binding protein Che-1 ELISA Kit;Human CHE1 ELISA Kit;Human DED ELISA Kit;Human BFR2 ELISA Kit;Human CHE-1 ELISA Kit;Human apoptosis antagonizing transcription factor ELISA Kit;Human protein AATF ELISA Kit;

  • Catalog
    E000151
  • species
    Human
  • GeneAATF
  • Other Species Mouse Aatf ELISA KitChicken AATF ELISA KitRat Aatf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AATF. No significant cross-reactivity or interference between Human AATF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human AATF and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseHuman AATF ELISA Kit allows for the in vitro quantitative determination of AATF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AATF in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AATF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human AATF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AATF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AATF. You can calculate the concentration of Human AATF in the samples by comparing the OD of the samples to the standard curve.
  • Human Protein AATF Protein information
  • Uniprot ID AATF_HUMAN
  • Uniprot AC Q9NY61; A6NCJ6; B3KQ26; Q9P0A4; Q9UNX5;
  • UniGene Hs.195740;
  • GeneID 26574
  • KEGG hsa:26574;
  • Human Protein AATF Protein SEQUENCE
  • SEQUENCE 560 AA; 63133 MW; EC493EF3B4C3A199 CRC64;

    MAGPQPLALQ LEQLLNPRPS EADPEADPEE ATAARVIDRF DEGEDGEGDF

    LVVGSIRKLA SASLLDTDKR YCGKTTSRKA WNEDHWEQTL PGSSDEEISD

    EEGSGDEDSE GLGLEEYDED DLGAAEEQEC GDHRESKKSR SHSAKTPGFS

    VQSISDFEKF TKGMDDLGSS EEEEDEESGM EEGDDAEDSQ GESEEDRAGD

    RNSEDDGVVM TFSSVKVSEE VEKGRAVKNQ IALWDQLLEG RIKLQKALLT

    TNQLPQPDVF PLFKDKGGPE FSSALKNSHK ALKALLRSLV GLQEELLFQY

    PDTRYLVDGT KPNAGSEEIS SEDDELVEEK KQQRRRVPAK RKLEMEDYPS

    FMAKRFADFT VYRNRTLQKW HDKTKLASGK LGKGFGAFER SILTQIDHIL

    MDKERLLRRT QTKRSVYRVL GKPEPAAQPV PESLPGEPEI LPQAPANAHL

    KDLDEEIFDD DDFYHQLLRE LIERKTSSLD PNDQVAMGRQ WLAIQKLRSK

    IHKKVDRKAS KGRKLRFHVL SKLLSFMAPI DHTTMNDDAR TELYRSLFGQ

    LHPPDEGHGD

  • UCSC uc002hni.3; human.;


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