Human FNDC4 ELISA Kit

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  • Alternative name

    Human fibronectin type III repeat-containing protein 1 ELISA Kit;Human FRCP1 ELISA Kit;Human fibronectin type III domain containing 4 ELISA Kit;Human fibronectin type III domain-containing protein 4 ELISA Kit;

  • Catalog
    E015073
  • species
    Human
  • GeneFNDC4
  • Standard CurveHuman FNDC4 ELISA Kit
  • Other Species Mouse Fndc4 ELISA KitBovine FNDC4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FNDC4. No significant cross-reactivity or interference between FNDC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FNDC4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman FNDC4 ELISA Kit allows for the in vitro quantitative determination of FNDC4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This FNDC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FNDC4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: FNDC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FNDC4 antibody and an FNDC4-HRP conjugate. The assay sample and buffer are incubated together with FNDC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FNDC4 concentration since FNDC4 from samples and FNDC4-HRP conjugate compete for the anti-FNDC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by FNDC4 from the sample, fewer sites are left to bind FNDC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FNDC4 concentration in each sample is interpolated from this standard curve.
  • Human Fibronectin type III domain-containing protein 4 Protein information
  • Uniprot ID FNDC4_HUMAN
  • Uniprot AC Q9H6D8; D6W560;
  • UniGene Hs.27836; Hs.732184;
  • GeneID 64838
  • KEGG hsa:64838;
  • Human Fibronectin type III domain-containing protein 4 Protein SEQUENCE
  • SEQUENCE 234 AA; 25159 MW; 5BCD23C12F583727 CRC64;

    MPSGCHSSPP SGLRGDMASL VPLSPYLSPT VLLLVSCDLG FVRADRPPSP

    VNVTVTHLRA NSATVSWDVP EGNIVIGYSI SQQRQNGPGQ RVIREVNTTT

    RACALWGLAE DSDYTVQVRS IGLRGESPPG PRVHFRTLKG SDRLPSNSSS

    PGDITVEGLD GERPLQTGEV VIIVVVLLMW AAVIGLFCRQ YDIIKDNDSN

    NNPKEKGKGP EQSPQGRPVG TRQKKSPSIN TIDV

  • UCSC uc002rkx.4; human.;




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