Human FNDC4 ELISA Kit

Alternative name
Human fibronectin type III repeat-containing protein 1 ELISA Kit;Human FRCP1 ELISA Kit;Human fibronectin type III domain containing 4 ELISA Kit;Human fibronectin type III domain-containing protein 4 ELISA Kit;
Catalog
E015073
species
Human
GeneFNDC4
Standard Curve
Other Species Mouse Fndc4 ELISA Kit Bovine FNDC4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of FNDC4. No significant cross-reactivity or interference between FNDC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FNDC4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman FNDC4 ELISA Kit allows for the in vitro quantitative determination of FNDC4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This FNDC4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FNDC4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: FNDC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FNDC4 antibody and an FNDC4-HRP conjugate. The assay sample and buffer are incubated together with FNDC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FNDC4 concentration since FNDC4 from samples and FNDC4-HRP conjugate compete for the anti-FNDC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by FNDC4 from the sample, fewer sites are left to bind FNDC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FNDC4 concentration in each sample is interpolated from this standard curve.
Human Fibronectin type III domain-containing protein 4 Protein information
Uniprot ID FNDC4_HUMAN
Uniprot AC Q9H6D8; D6W560;
UniGene Hs.27836; Hs.732184;
GeneID 64838
KEGG hsa:64838;
Human Fibronectin type III domain-containing protein 4 Protein SEQUENCE
SEQUENCE 234 AA; 25159 MW; 5BCD23C12F583727 CRC64;
MPSGCHSSPP SGLRGDMASL VPLSPYLSPT VLLLVSCDLG FVRADRPPSP
VNVTVTHLRA NSATVSWDVP EGNIVIGYSI SQQRQNGPGQ RVIREVNTTT
RACALWGLAE DSDYTVQVRS IGLRGESPPG PRVHFRTLKG SDRLPSNSSS
PGDITVEGLD GERPLQTGEV VIIVVVLLMW AAVIGLFCRQ YDIIKDNDSN
NNPKEKGKGP EQSPQGRPVG TRQKKSPSIN TIDV
UCSC uc002rkx.4; human.;

Category

Human FNDC4 ELISA Kit

Human fibronectin type III repeat-containing protein 1 ELISA Kit;Human FRCP1 ELISA Kit;Human fibronectin type III domain containing 4 ELISA Kit;Human fibronectin type III domain-containing protein 4 ELISA Kit;