Human FGFBP2 ELISA Kit

Alternative name
Human FGF-BP2 ELISA Kit;Human FGF-binding protein 2 ELISA Kit;Human FGFBP-2 ELISA Kit;Human 37 kDa killer-specific secretory protein ELISA Kit;Human KSP37 ELISA Kit;Human HBp17-related protein ELISA Kit;Human HBp17-RP ELISA Kit;Human HBP17RP ELISA Kit;Human fibroblast growth factor binding protein 2 ELISA Kit;Human fibroblast growth factor-binding protein 2 ELISA Kit;Human killer-specific secretory protein of 37 kDa ELISA Kit;
Catalog
E014991
species
Human
GeneFGFBP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of FGFBP2. No significant cross-reactivity or interference between FGFBP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FGFBP2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman FGFBP2 ELISA Kit allows for the in vitro quantitative determination of FGFBP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This FGFBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FGFBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||FGFBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGFBP2 antibody and an FGFBP2-HRP conjugate. The assay sample and buffer are incubated together with FGFBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGFBP2 concentration since FGFBP2 from samples and FGFBP2-HRP conjugate compete for the anti-FGFBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGFBP2 from the sample, fewer sites are left to bind FGFBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGFBP2 concentration in each sample is interpolated from this standard curve.
Human Fibroblast growth factor-binding protein 2 Protein information
Uniprot ID FGFP2_HUMAN
Uniprot AC Q9BYJ0;
UniGene Hs.98785;
GeneID 83888
KEGG hsa:83888;
Human Fibroblast growth factor-binding protein 2 Protein SEQUENCE
SEQUENCE 223 AA; 24581 MW; 792E68933E7929D9 CRC64;
MKFVPCLLLV TLSCLGTLGQ APRQKQGSTG EEFHFQTGGR DSCTMRPSSL
GQGAGEVWLR VDCRNTDQTY WCEYRGQPSM CQAFAADPKP YWNQALQELR
RLHHACQGAP VLRPSVCREA GPQAHMQQVT SSLKGSPEPN QQPEAGTPSL
RPKATVKLTE ATQLGKDSME ELGKAKPTTR PTAKPTQPGP RPGGNEEAKK
KAWEHCWKPF QALCAFLISF FRG
UCSC uc003gon.4; human.;

Human FGFBP2 ELISA Kit

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