Human FGFBP2 ELISA Kit

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  • Alternative name

    Human FGF-BP2 ELISA Kit;Human FGF-binding protein 2 ELISA Kit;Human FGFBP-2 ELISA Kit;Human 37 kDa killer-specific secretory protein ELISA Kit;Human KSP37 ELISA Kit;Human HBp17-related protein ELISA Kit;Human HBp17-RP ELISA Kit;Human HBP17RP ELISA Kit;Human fibroblast growth factor binding protein 2 ELISA Kit;Human fibroblast growth factor-binding protein 2 ELISA Kit;Human killer-specific secretory protein of 37 kDa ELISA Kit;

  • Catalog
    E014991
  • species
    Human
  • GeneFGFBP2
  • Standard CurveHuman FGFBP2 ELISA Kit
  • Other Species Mouse FGFBP2 ELISA KitChicken FGFBP2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FGFBP2. No significant cross-reactivity or interference between FGFBP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FGFBP2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman FGFBP2 ELISA Kit allows for the in vitro quantitative determination of FGFBP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This FGFBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FGFBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||FGFBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGFBP2 antibody and an FGFBP2-HRP conjugate. The assay sample and buffer are incubated together with FGFBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGFBP2 concentration since FGFBP2 from samples and FGFBP2-HRP conjugate compete for the anti-FGFBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGFBP2 from the sample, fewer sites are left to bind FGFBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGFBP2 concentration in each sample is interpolated from this standard curve.
  • Human Fibroblast growth factor-binding protein 2 Protein information
  • Uniprot ID FGFP2_HUMAN
  • Uniprot AC Q9BYJ0;
  • UniGene Hs.98785;
  • GeneID 83888
  • KEGG hsa:83888;
  • Human Fibroblast growth factor-binding protein 2 Protein SEQUENCE
  • SEQUENCE 223 AA; 24581 MW; 792E68933E7929D9 CRC64;

    MKFVPCLLLV TLSCLGTLGQ APRQKQGSTG EEFHFQTGGR DSCTMRPSSL

    GQGAGEVWLR VDCRNTDQTY WCEYRGQPSM CQAFAADPKP YWNQALQELR

    RLHHACQGAP VLRPSVCREA GPQAHMQQVT SSLKGSPEPN QQPEAGTPSL

    RPKATVKLTE ATQLGKDSME ELGKAKPTTR PTAKPTQPGP RPGGNEEAKK

    KAWEHCWKPF QALCAFLISF FRG

  • UCSC uc003gon.4; human.;


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