Human FGF1 ELISA Kit

Alternative name
Human FGF-1 ELISA Kit;Human Acidic fibroblast growth factor ELISA Kit;Human AFGF ELISA Kit;Human Endothelial cell growth factor ELISA Kit;Human ECGF ELISA Kit;Human heparin-binding growth factor 1 ELISA Kit;Human HBGF-1 ELISA Kit;Human FGFA ELISA Kit;Human ECGF-beta ELISA Kit;Human ECGFA ELISA Kit;Human ECGFB ELISA Kit;Human FGF-alpha ELISA Kit;Human GLIO703 ELISA Kit;Human HBGF1 ELISA Kit;Human fibroblast growth factor 1 ELISA Kit;Human beta-endothelial cell growth factor ELISA Kit;Human endothelial cell growth factor, alpha ELISA Kit;Human endothelial cell growth factor, beta ELISA Kit;Human fibroblast growth factor 1 (acidic) ELISA Kit;
Catalog
E014888
species
Human
GeneFGF1
Standard Curve
Other Species Human AFGF/FGF1 ELISA Kit Mouse AFGF/FGF1 ELISA Kit Mouse Fgf1 ELISA Kit Bovine FGF1 ELISA Kit Canine FGF1 ELISA Kit Chicken FGF1 ELISA Kit Porcine FGF1 ELISA Kit Rat Fgf1 ELISA Kit Sheep FGF1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1). No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 6.0pg/mL.
Intended UseHuman FGF1 ELISA Kit allows for the in vitro quantitative determination of FGF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 1, Acidic (FGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Fibroblast growth factor 1 Protein information
Uniprot ID FGF1_HUMAN
Uniprot AC P05230; B2R5T0; D3DQF2; P07502; Q16588;
UniGene Hs.483635;
GeneID 2246
KEGG hsa:2246;
Human Fibroblast growth factor 1 Protein SEQUENCE
SEQUENCE 155 AA; 17460 MW; F586E8BFB09F1580 CRC64;
MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR
DRSDQHIQLQ LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF
LERLEENHYN TYISKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL
PVSSD
UCSC uc003lmm.5; human. [P05230-1];

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Human FGF1 ELISA Kit