Human FAP ELISA Kit

Alternative name
Human 170 kDa melanoma membrane-bound gelatinase ELISA Kit;Human Dipeptidyl peptidase FAP ELISA Kit;Human fibroblast activation protein alpha ELISA Kit;Human FAPalpha ELISA Kit;Human Gelatine degradation protease FAP ELISA Kit;Human integral membrane serine protease ELISA Kit;Human Post-proline cleaving enzyme ELISA Kit;Human Serine integral membrane protease ELISA Kit;Human SIMP ELISA Kit;Human surface-expressed protease ELISA Kit;Human seprase ELISA Kit;Human APCE ELISA Kit;Human DPPIV ELISA Kit;Human FAPA ELISA Kit;Human prolyl endopeptidase FAP ELISA Kit;Human dipeptidyl peptidase FAP ELISA Kit;Human gelatine degradation protease FAP ELISA Kit;Human post-proline cleaving enzyme ELISA Kit;
Catalog
E014884
species
Human
GeneFAP
Other Species Mouse Fap ELISA Kit Bovine Antiplasmin-cleaving enzyme FAP, soluble form ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human FAP. No significant cross-reactivity or interference between human FAP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human FAP is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman FAP ELISA Kit allows for the in vitro quantitative determination of FAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Antiplasmin-cleaving enzyme FAP, soluble form Protein information
Uniprot ID SEPR_HUMAN
Uniprot AC Q12884; O00199; Q53TP5; Q86Z29; Q99998; Q9UID4;
UniGene Hs.654370;
GeneID 2191
KEGG hsa:2191;
Human Antiplasmin-cleaving enzyme FAP, soluble form Protein SEQUENCE
SEQUENCE 760 AA; 87713 MW; 7FF817B5A4F75142 CRC64;
MKTWVKIVFG VATSAVLALL VMCIVLRPSR VHNSEENTMR ALTLKDILNG
TFSYKTFFPN WISGQEYLHQ SADNNIVLYN IETGQSYTIL SNRTMKSVNA
SNYGLSPDRQ FVYLESDYSK LWRYSYTATY YIYDLSNGEF VRGNELPRPI
QYLCWSPVGS KLAYVYQNNI YLKQRPGDPP FQITFNGREN KIFNGIPDWV
YEEEMLATKY ALWWSPNGKF LAYAEFNDTD IPVIAYSYYG DEQYPRTINI
PYPKAGAKNP VVRIFIIDTT YPAYVGPQEV PVPAMIASSD YYFSWLTWVT
DERVCLQWLK RVQNVSVLSI CDFREDWQTW DCPKTQEHIE ESRTGWAGGF
FVSTPVFSYD AISYYKIFSD KDGYKHIHYI KDTVENAIQI TSGKWEAINI
FRVTQDSLFY SSNEFEEYPG RRNIYRISIG SYPPSKKCVT CHLRKERCQY
YTASFSDYAK YYALVCYGPG IPISTLHDGR TDQEIKILEE NKELENALKN
IQLPKEEIKK LEVDEITLWY KMILPPQFDR SKKYPLLIQV YGGPCSQSVR
SVFAVNWISY LASKEGMVIA LVDGRGTAFQ GDKLLYAVYR KLGVYEVEDQ
ITAVRKFIEM GFIDEKRIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV
SSWEYYASVY TERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT
ADDNVHFQNS AQIAKALVNA QVDFQAMWYS DQNHGLSGLS TNHLYTHMTH
FLKQCFSLSD
UCSC uc002ucd.3; human. [Q12884-1];

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Category

Human FAP ELISA Kit