Human FGF2 ELISA Kit

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  • Alternative name

    Human FGF-2 ELISA Kit;Human Basic fibroblast growth factor ELISA Kit;Human BFGF ELISA Kit;Human heparin-binding growth factor 2 ELISA Kit;Human HBGF-2 ELISA Kit;Human FGFB ELISA Kit;Human fibroblast growth factor 2 ELISA Kit;Human basic fibroblast growth factor bFGF ELISA Kit;Human fibroblast growth factor 2 (basic) ELISA Kit;Human prostatropin ELISA Kit;

  • Catalog
    E014765
  • species
    Human
  • GeneFGF2
  • Standard CurveHuman FGF2 ELISA Kit
  • Other Species Human bFGF / FGF2 ELISA KitHuman bFGF/FGF2 ELISA KitMouse bFGF / FGF2 ELISA KitMouse bFGF/FGF2 ELISA KitMouse Fgf2 ELISA KitBovine FGF2 ELISA KitGuinea Pig FGF2 ELISA KitChicken FGF2 ELISA KitRabbit FGF2 ELISA KitRat Fgf2 ELISA KitSheep FGF2 ELISA Kit
  • SpecificityThe Human FGF2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human FGF2 proteins within the range of 7.8 pg/ml - 500 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human FGF2 is typically less than 4 pg/ml.
  • Intended UseHuman FGF2 ELISA Kit allows for the in vitro quantitative determination of FGF2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The Human FGF2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF2 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human FGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: Human FGF-Basic or Fibroblast Growth Factor 2, also known commonly as HBGF-2 or Heparin-Binding Growth Factor 2, is a 288 amino acid cytokine protein encoded by the FGF2gene at locus 4q26 on chromosome 4. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, would healing and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The monomeric FGF-Basic protein interacts with many other proteins such as CSPG4 and FGFBP1 while also believed to be found in a complex with FGFBP1, FGF1 and FGF2. Expression patterns for this protein typically take place in granulosa and cumulus cells, but FGF2 is also found in hepatocellular carcinoma cells (not non-cancerous liver tissue). Several experiments were performed on FGF-Basic to determine its pH and temperature properties. It has been recently determined that FGF-Basic retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20 degree C, while being inactivated after 3 minutes at 60 degree C or 1 minute at 80 degree C.
  • Human Fibroblast growth factor 2 Protein information
  • Uniprot ID FGF2_HUMAN
  • Uniprot AC Q9UC54; Q9UCS5; Q9UCS6;
  • UniGene Hs.284244;
  • GeneID 2247
  • KEGG hsa:2247;
  • Human Fibroblast growth factor 2 Protein SEQUENCE
  • SEQUENCE 288 AA; 30770 MW; EAE98552A39D5E19 CRC64;

    MVGVGGGDVE DVTPRPGGCQ ISGRGARGCN GIPGAAAWEA ALPRRRPRRH

    PSVNPRSRAA GSPRTRGRRT EERPSGSRLG DRGRGRALPG GRLGGRGRGR

    APERVGGRGR GRGTAAPRAA PAARGSRPGP AGTMAAGSIT TLPALPEDGG

    SGAFPPGHFK DPKRLYCKNG GFFLRIHPDG RVDGVREKSD PHIKLQLQAE

    ERGVVSIKGV CANRYLAMKE DGRLLASKCV TDECFFFERL ESNNYNTYRS

    RKYTSWYVAL KRTGQYKLGS KTGPGQKAIL FLPMSAKS

  • UCSC uc062zki.1; human. [P09038-4];




tag

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