Human ACTN4 ELISA Kit

Contact Us
  • Alternative name

    Human non-muscle alpha-actinin 4 ELISA Kit;Human ACTININ-4 ELISA Kit;Human FSGS ELISA Kit;Human FSGS1 ELISA Kit;Human actinin alpha 4 ELISA Kit;Human alpha-actinin-4 ELISA Kit;Human focal segmental glomerulosclerosis 1 ELISA Kit;

  • Catalog
    E001473
  • species
    Human
  • GeneACTN4
  • Other Species Mouse Actn4 ELISA KitBovine ACTN4 ELISA KitChicken ACTN4 ELISA KitRat Actn4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACTN 4. No significant cross-reactivity or interference between ACTN 4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTN 4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ACTN4 ELISA Kit allows for the in vitro quantitative determination of ACTN4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Intended Uses: This ACTN 4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACTN 4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACTN 4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTN 4 antibody and an ACTN 4-HRP conjugate. The assay sample and buffer are incubated together with ACTN 4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN 4 concentration since ACTN 4 from samples and ACTN 4-HRP conjugate compete for the anti-ACTN 4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN 4 from the sample, fewer sites are left to bind ACTN 4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN 4 concentration in each sample is interpolated from this standard curve.
  • Human Alpha-actinin-4 Protein information
  • Uniprot ID ACTN4_HUMAN
  • Uniprot AC O43707; A4K467; D6PXK4; O76048;
  • UniGene Hs.270291;
  • GeneID 81
  • KEGG hsa:81;
  • Human Alpha-actinin-4 Protein SEQUENCE
  • SEQUENCE 911 AA; 104854 MW; 461580C3F22937D1 CRC64;

    MVDYHAANQS YQYGPSSAGN GAGGGGSMGD YMAQEDDWDR DLLLDPAWEK

    QQRKTFTAWC NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE

    RGKMRVHKIN NVNKALDFIA SKGVKLVSIG AEEIVDGNAK MTLGMIWTII

    LRFAIQDISV EETSAKEGLL LWCQRKTAPY KNVNVQNFHI SWKDGLAFNA

    LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA

    RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYEK

    LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK

    CQLEINFNTL QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW

    LLNEIRRLER LDHLAEKFRQ KASIHEAWTD GKEAMLKHRD YETATLSDIK

    ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL NELDYYDSHN VNTRCQKICD

    QWDALGSLTH SRREALEKTE KQLEAIDQLH LEYAKRAAPF NNWMESAMED

    LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES

    NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL

    RRQFASQANV VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY

    KPNLDLLEQQ HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV

    ENQILTRDAK GISQEQMQEF RASFNHFDKD HGGALGPEEF KACLISLGYD

    VENDRQGEAE FNRIMSLVDP NHSGLVTFQA FIDFMSRETT DTDTADQVIA

    SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS

    FSTALYGESD L

  • UCSC uc002oja.3; human. [O43707-1];


Related products with Human ACTN4 ELISA Kit


tag

Inquiry About Human ACTN4 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!