Human ACTN4 ELISA Kit

Alternative name
Human non-muscle alpha-actinin 4 ELISA Kit;Human ACTININ-4 ELISA Kit;Human FSGS ELISA Kit;Human FSGS1 ELISA Kit;Human actinin alpha 4 ELISA Kit;Human alpha-actinin-4 ELISA Kit;Human focal segmental glomerulosclerosis 1 ELISA Kit;
Catalog
E001473
species
Human
GeneACTN4
Other Species Mouse Actn4 ELISA Kit Bovine ACTN4 ELISA Kit Chicken ACTN4 ELISA Kit Rat Actn4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACTN 4. No significant cross-reactivity or interference between ACTN 4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTN 4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ACTN4 ELISA Kit allows for the in vitro quantitative determination of ACTN4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalIntended Uses: This ACTN 4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACTN 4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACTN 4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTN 4 antibody and an ACTN 4-HRP conjugate. The assay sample and buffer are incubated together with ACTN 4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN 4 concentration since ACTN 4 from samples and ACTN 4-HRP conjugate compete for the anti-ACTN 4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN 4 from the sample, fewer sites are left to bind ACTN 4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN 4 concentration in each sample is interpolated from this standard curve.
Human Alpha-actinin-4 Protein information
Uniprot ID ACTN4_HUMAN
Uniprot AC O43707; A4K467; D6PXK4; O76048;
UniGene Hs.270291;
GeneID 81
KEGG hsa:81;
Human Alpha-actinin-4 Protein SEQUENCE
SEQUENCE 911 AA; 104854 MW; 461580C3F22937D1 CRC64;
MVDYHAANQS YQYGPSSAGN GAGGGGSMGD YMAQEDDWDR DLLLDPAWEK
QQRKTFTAWC NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE
RGKMRVHKIN NVNKALDFIA SKGVKLVSIG AEEIVDGNAK MTLGMIWTII
LRFAIQDISV EETSAKEGLL LWCQRKTAPY KNVNVQNFHI SWKDGLAFNA
LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA
RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYEK
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK
CQLEINFNTL QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW
LLNEIRRLER LDHLAEKFRQ KASIHEAWTD GKEAMLKHRD YETATLSDIK
ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL NELDYYDSHN VNTRCQKICD
QWDALGSLTH SRREALEKTE KQLEAIDQLH LEYAKRAAPF NNWMESAMED
LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES
NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL
RRQFASQANV VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY
KPNLDLLEQQ HQLIQEALIF DNKHTNYTME HIRVGWEQLL TTIARTINEV
ENQILTRDAK GISQEQMQEF RASFNHFDKD HGGALGPEEF KACLISLGYD
VENDRQGEAE FNRIMSLVDP NHSGLVTFQA FIDFMSRETT DTDTADQVIA
SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS
FSTALYGESD L
UCSC uc002oja.3; human. [O43707-1];

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Category

Human ACTN4 ELISA Kit

Human non-muscle alpha-actinin 4 ELISA Kit;Human ACTININ-4 ELISA Kit;Human FSGS ELISA Kit;Human FSGS1 ELISA Kit;Human actinin alpha 4 ELISA Kit;Human alpha-actinin-4 ELISA Kit;Human focal segmental glomerulosclerosis 1 ELISA Kit;