SpecificityThis assay has high sensitivity and excellent specificity for detection of PRE-?2 MG. No significant cross-reactivity or interference between PRE-?2 MG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRE-?2 MG and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman Pre Alpha 2 MG ELISA Kit allows for the in vitro quantitative determination of Pre Alpha 2 MG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilySignal Transduction
Product Description specificalPrinciple of the assay: PRE-?2 MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRE-?2 MG antibody and an PRE-?2 MG-HRP conjugate. The assay sample and buffer are incubated together with PRE-?2 MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRE-?2 MG concentration since PRE-?2 MG from samples and PRE-?2 MG-HRP conjugate compete for the anti-PRE-?2 MG antibody binding site. Since the number of sites is limited, as more sites are occupied by PRE-?2 MG from the sample, fewer sites are left to bind PRE-?2 MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRE-?2 MG concentration in each sample is interpolated from this standard curve.