Human FBL ELISA Kit

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  • Alternative name

    Human 34 kDa nucleolar scleroderma antigen ELISA Kit;Human histone-glutamine methyltransferase ELISA Kit;Human FIB1 ELISA Kit;Human FLRN ELISA Kit;Human FIB ELISA Kit;Human RNU3IP1 ELISA Kit;Human fibrillarin ELISA Kit;Human rRNA 2'-O-methyltransferase fibrillarin ELISA Kit;Human 34-kD nucleolar scleroderma antigen ELISA Kit;Human RNA, U3 small nucleolar interacting protein 1 ELISA Kit;

  • Catalog
    E014574
  • species
    Human
  • GeneFBL
  • Other Species Mouse Fbl ELISA KitRat Fbl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human FBL. No significant cross-reactivity or interference between Human FBL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human FBL and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.938ng/mL
  • Intended UseHuman FBL ELISA Kit allows for the in vitro quantitative determination of FBL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FBL in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FBL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human FBL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FBL. You can calculate the concentration of Human FBL in the samples by comparing the OD of the samples to the standard curve.
  • Human rRNA 2'-O-methyltransferase fibrillarin Protein information
  • Uniprot ID FBRL_HUMAN
  • Uniprot AC P22087; B5BUE8; O75259; Q6IAT5; Q9UPI6;
  • UniGene Hs.299002;
  • GeneID 2091
  • KEGG hsa:2091;
  • Human rRNA 2'-O-methyltransferase fibrillarin Protein SEQUENCE
  • SEQUENCE 321 AA; 33784 MW; 2123F41E01EC557A CRC64;

    MKPGFSPRGG GFGGRGGFGD RGGRGGRGGF GGGRGRGGGF RGRGRGGGGG

    GGGGGGGGRG GGGFHSGGNR GRGRGGKRGN QSGKNVMVEP HRHEGVFICR

    GKEDALVTKN LVPGESVYGE KRVSISEGDD KIEYRAWNPF RSKLAAAILG

    GVDQIHIKPG AKVLYLGAAS GTTVSHVSDI VGPDGLVYAV EFSHRSGRDL

    INLAKKRTNI IPVIEDARHP HKYRMLIAMV DVIFADVAQP DQTRIVALNA

    HTFLRNGGHF VISIKANCID STASAEAVFA SEVKKMQQEN MKPQEQLTLE

    PYERDHAVVV GVYRPPPKVK N

  • UCSC uc002omn.4; human.;


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