Human FSCN1 ELISA Kit

Alternative name
Human 55 kDa actin-bundling protein ELISA Kit;Human Singed-like protein ELISA Kit;Human p55 ELISA Kit;Human FAN1 ELISA Kit;Human HSN ELISA Kit;Human SNL ELISA Kit;Human fascin actin-bundling protein 1 ELISA Kit;Human fascin ELISA Kit;Human fascin homolog 1, actin-bundling protein ELISA Kit;Human singed-like (fascin homolog, sea urchin) ELISA Kit;
Catalog
E014467
species
Human
GeneFSCN1
Other Species Mouse Fscn1 ELISA Kit Rat Fscn1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseHuman FSCN1 ELISA Kit allows for the in vitro quantitative determination of FSCN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FASCIN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FASCIN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FASCIN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FASCIN can be calculated.
Human Fascin Protein information
Uniprot ID FSCN1_HUMAN
Uniprot AC Q16658; A6NI89; B2RE97; Q96IC5; Q96IH1; Q9BRF1;
UniGene Hs.118400;
GeneID 6624
KEGG hsa:6624;
Human Fascin Protein SEQUENCE
SEQUENCE 493 AA; 54530 MW; C1453714BED6109A CRC64;
MTANGTAEAV QIQFGLINCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ
PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPGPDCR FLIVAHDDGR
WSLQSEAHRR YFGGTEDRLS CFAQTVSPAE KWSVHIAMHP QVNIYSVTRK
RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT ADHRFLRHDG
RLVARPEPAT GYTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK
DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR
DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS
NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV
TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSA VTSSGDTPVD
FFFEFCDYNK VAIKVGGRYL KGDHAGVLKA SAETVDPASL WEY
UCSC uc003sou.4; human.;

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Human FSCN1 ELISA Kit