Human FBXO34 ELISA Kit

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  • Alternative name

    Human Fbx34 ELISA Kit;Human CGI-301 ELISA Kit;Human F-box protein 34 ELISA Kit;Human F-box only protein 34 ELISA Kit;Human protein CGI-301 ELISA Kit;

  • Catalog
    E013960
  • species
    Human
  • GeneFBXO34
  • Standard CurveHuman FBXO34 ELISA Kit
  • Other Species Mouse Fbxo34 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FBXO34. No significant cross-reactivity or interference between FBXO34 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBXO34 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman FBXO34 ELISA Kit allows for the in vitro quantitative determination of FBXO34 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: FBXO34 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBXO34 antibody and an FBXO34-HRP conjugate. The assay sample and buffer are incubated together with FBXO34-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXO34 concentration since FBXO34 from samples and FBXO34-HRP conjugate compete for the anti-FBXO34 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXO34 from the sample, fewer sites are left to bind FBXO34-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXO34 concentration in each sample is interpolated from this standard curve.
  • Human F-box only protein 34 Protein information
  • Uniprot ID FBX34_HUMAN
  • Uniprot AC GIEHCSVH YVSDSGDGVY AGRPLSVIQM VAFLEQRASA LLASCSKNCT NSPAIVRFSG
  • UniGene Hs.525348; Hs.654725;
  • GeneID 55030
  • KEGG hsa:55030;
  • Human F-box only protein 34 Protein SEQUENCE
  • SEQUENCE 711 AA; 78711 MW; 6D289A9320AE9C42 CRC64;

    MHLKPYWKLQ KKEHPPEVSR ETQRTPMNHQ KAVNDETCKA SHITSSVFPS

    ASLGKASSRK PFGILSPNVL CSMSGKSPVE SSLNVKTKKN APSATIHQGE

    EEGPLDIWAV VKPGNTKEKI AFFASHQCSN RIGSMKIKSS WDIDGRATKR

    RKKSGDLKKA KVQVERMREV NSRCYQPEPF ACGIEHCSVH YVSDSGDGVY

    AGRPLSVIQM VAFLEQRASA LLASCSKNCT NSPAIVRFSG QSRGVPAVSE

    SYSAPGACEE PTERGNLEVG EPQSEPVRVL DMVAKLESEC LKRQGQREPG

    SLSRNNSFRR NVGRVLLANS TQADEGKTKK GVLEAPDTQV NPVGSVSVDC

    GPSRADRCSP KEDQAWDGAS QDCPPLPAGV SFHIDSAELE PGSQTAVKNS

    NRYDVEMTDE LVGLPFSSHT YSQASELPTD AVDCMSRELV SLTSRNPDQR

    KESLCISITV SKVDKDQPSI LNSCEDPVPG MLFFLPPGQH LSDYSQLNES

    TTKESSEASQ LEDAAGGDSA SEEKSGSAEP FVLPASSVES TLPVLEASSW

    KKQVSHDFLE TRFKIQQLLE PQQYMAFLPH HIMVKIFRLL PTKSLVALKC

    TCCYFKFIIE YYNIRPADSR WVRDPRYRED PCKQCKKKYV KGDVSLCRWH

    PKPYCQALPY GPGYWMCCHR SQKGFPGCKL GLHDNHWVPA CHSFNRAIHK

    KAKGTEAEEE Y

  • UCSC uc001xbu.4; human.;



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