Human ALPPL2 ELISA Kit

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  • Alternative name

    Human ALP-1 ELISA Kit;Human alkaline phosphatase Nagao isozyme ELISA Kit;Human germ cell alkaline phosphatase ELISA Kit;Human GCAP ELISA Kit;Human Placental alkaline phosphatase-like ELISA Kit;Human PLAP-like ELISA Kit;Human ALPPL ELISA Kit;Human ALPG ELISA Kit;Human alkaline phosphatase, placental like 2 ELISA Kit;Human alkaline phosphatase, placental-like ELISA Kit;Human Nagao isozyme ELISA Kit;Human placental-like alkaline phosphatase ELISA Kit;Human testicular and thymus alkaline phosphatase ELISA Kit;

  • Catalog
    E001392
  • species
    Human
  • GeneALPPL2
  • Standard CurveHuman ALPPL2 ELISA Kit
  • Other Species Mouse Alppl2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). No significant cross-reactivity or interference between Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.113ng/mL.
  • Intended UseHuman ALPPL2 ELISA Kit allows for the in vitro quantitative determination of ALPPL2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Alkaline phosphatase, placental-like Protein information
  • Uniprot ID PPBN_HUMAN
  • Uniprot AC P10696; A8KAF2; Q16727; Q53S81; Q96CM1;
  • UniGene Hs.333509;
  • GeneID 251
  • KEGG hsa:251;
  • Human Alkaline phosphatase, placental-like Protein SEQUENCE
  • SEQUENCE 532 AA; 57377 MW; 25EB56C901B61505 CRC64;

    MQGPWVLLLL GLRLQLSLGI IPVEEENPDF WNRQAAEALG AAKKLQPAQT

    AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK

    TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS

    VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE

    GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK

    NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD

    STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGRIDHGHHE SRAYRALTET

    IMFDDAIERA GQLTSEEDTL SLVTADHSHV FSFGGYPLRG SSIFGLAPGK

    ARDRKAYTVL LYGNGPGYVL KDGARPDVTE SESGSPEYRQ QSAVPLDGET

    HAGEDVAVFA RGPQAHLVHG VQEQTFIAHV MAFAACLEPY TACDLAPRAG

    TTDAAHPGPS VVPALLPLLA GTLLLLGTAT AP

  • UCSC uc002vss.5; human.;


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