Human SOD3 ELISA Kit

Alternative name
Human EC-SOD ELISA Kit;Human superoxide dismutase 3 ELISA Kit;Human extracellular superoxide dismutase [Cu-Zn] ELISA Kit;Human superoxide dismutase 3, extracellular ELISA Kit;Human testicular tissue protein Li 175 ELISA Kit;
Catalog
E013913
species
Human
GeneSOD3
SpecificityThis assay has high sensitivity and excellent specificity for detection of SOD3 . No significant cross-reactivity or interference between SOD3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 3.75ng/ml
Intended UseHuman SOD3 ELISA Kit allows for the in vitro quantitative determination of SOD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SOD3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SOD3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SOD3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SOD3 can be calculated.
Human Extracellular superoxide dismutase [Cu-Zn] Protein information
Uniprot ID SODE_HUMAN
Uniprot AC P08294; Q5U781; Q6FHA2;
UniGene Hs.2420;
GeneID 6649
KEGG hsa:6649;
Human Extracellular superoxide dismutase [Cu-Zn] Protein SEQUENCE
SEQUENCE 240 AA; 25851 MW; 585B8DEBFC506CF4 CRC64;
MLALLCSCLL LAAGASDAWT GEDSAEPNSD SAEWIRDMYA KVTEIWQEVM
QRRDDDGALH AACQVQPSAT LDAAQPRVTG VVLFRQLAPR AKLDAFFALE
GFPTEPNSSS RAIHVHQFGD LSQGCESTGP HYNPLAVPHP QHPGDFGNFA
VRDGSLWRYR AGLAASLAGP HSIVGRAVVV HAGEDDLGRG GNQASVENGN
AGRRLACCVV GVCGPGLWER QAREHSERKK RRRESECKAA
UCSC uc003gqz.4; human.;

Category

Human SOD3 ELISA Kit

Human EC-SOD ELISA Kit;Human superoxide dismutase 3 ELISA Kit;Human extracellular superoxide dismutase [Cu-Zn] ELISA Kit;Human superoxide dismutase 3, extracellular ELISA Kit;Human testicular tissue protein Li 175 ELISA Kit;