Human ECM1 ELISA Kit

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  • Alternative name

    Human secretory component p85 ELISA Kit;Human URBWD ELISA Kit;Human extracellular matrix protein 1 ELISA Kit;Human testicular tissue protein Li 61 ELISA Kit;

  • Catalog
    E013892
  • species
    Human
  • GeneECM1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ECM1. No significant cross-reactivity or interference between human ECM1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ECM1 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman ECM1 ELISA Kit allows for the in vitro quantitative determination of ECM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ECM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ECM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Extracellular matrix protein 1 Protein information
  • Uniprot ID ECM1_HUMAN
  • Uniprot AC Q8IZ60;
  • UniGene Hs.81071;
  • GeneID 1893
  • KEGG hsa:1893;
  • Human Extracellular matrix protein 1 Protein SEQUENCE
  • SEQUENCE 540 AA; 60674 MW; EA575895CDE068BE CRC64;

    MGTTARAALV LTYLAVASAA SEGGFTATGQ RQLRPEHFQE VGYAAPPSPP

    LSRSLPMDHP DSSQHGPPFE GQSQVQPPPS QEATPLQQEK LLPAQLPAEK

    EVGPPLPQEA VPLQKELPSL QHPNEQKEGT PAPFGDQSHP EPESWNAAQH

    CQQDRSQGGW GHRLDGFPPG RPSPDNLNQI CLPNRQHVVY GPWNLPQSSY

    SHLTRQGETL NFLEIGYSRC CHCRSHTNRL ECAKLVWEEA MSRFCEAEFS

    VKTRPHWCCT RQGEARFSCF QEEAPQPHYQ LRACPSHQPD ISSGLELPFP

    PGVPTLDNIK NICHLRRFRS VPRNLPATDP LQRELLALIQ LEREFQRCCR

    QGNNHTCTWK AWEDTLDKYC DREYAVKTHH HLCCRHPPSP TRDECFARRA

    PYPNYDRDIL TIDIGRVTPN LMGHLCGNQR VLTKHKHIPG LIHNMTARCC

    DLPFPEQACC AEEEKLTFIN DLCGPRRNIW RDPALCCYLS PGDEQVNCFN

    INYLRNVALV SGDTENAKGQ GEQGSTGGTN ISSTSEPKEE

  • UCSC uc001eus.4; human. [Q16610-1];



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