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Alternative name
Basigin ELISA KIT; 5F7 ELISA KIT; Collagenase stimulatory factor ELISA KIT; Extracellular matrix metalloproteinase inducer ELISA KIT; EMMPRIN ELISA KIT; Leukocyte activation antigen M6 ELISA KIT; OK blood group antigen ELISA KIT; Tumor cell-derived collagenase stimulatory factor ELISA KIT; TCSF ELISA KIT; CD_antigen: CD147BSG ELISA KIT; EMMPRIN ELISA KIT; TCSF ELISA KIT
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Catalog
E013886
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species
Human
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GeneEMMPRIN
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN).
No significant cross-reactivity or interference between Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 12.7pg/mL.
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Intended UseHuman EMMPRIN ELISA Kit allows for the in vitro quantitative determination of EMMPRIN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Matrix Metalloproteinase Inducer (EMMPRIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Matrix Metalloproteinase Inducer (EMMPRIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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