Human ALPP ELISA Kit

Alternative name
Human alkaline phosphatase Regan isozyme ELISA Kit;Human placental alkaline phosphatase 1 ELISA Kit;Human PLAP-1 ELISA Kit;Human PLAP ELISA Kit;Human ALP ELISA Kit;Human PALP ELISA Kit;Human alkaline phosphatase, placental ELISA Kit;Human alkaline phosphatase, placental type ELISA Kit;Human alkaline phosphomonoesterase ELISA Kit;Human glycerophosphatase ELISA Kit;
Catalog
E001388
species
Human
GeneALPP
Standard Curve
Other Species Human PLAP/ALPP ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ALPP. No significant cross-reactivity or interference between ALPP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of ALPP is typically less than 47pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two st
Intended UseHuman ALPP ELISA Kit allows for the in vitro quantitative determination of ALPP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ALPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alkaline phosphatase, placental type Protein information
Uniprot ID PPB1_HUMAN
Uniprot AC P05187; P05188; P06861; Q53S78; Q96DB7;
UniGene Hs.284255;
GeneID 250
KEGG hsa:250;
Human Alkaline phosphatase, placental type Protein SEQUENCE
SEQUENCE 535 AA; 57954 MW; 13C136679A70C76B CRC64;
MLGPCMLLLL LLLGLRLQLS LGIIPVEEEN PDFWNREAAE ALGAAKKLQP
AQTAAKNLII FLGDGMGVST VTAARILKGQ KKDKLGPEIP LAMDRFPYVA
LSKTYNVDKH VPDSGATATA YLCGVKGNFQ TIGLSAAARF NQCNTTRGNE
VISVMNRAKK AGKSVGVVTT TRVQHASPAG TYAHTVNRNW YSDADVPASA
RQEGCQDIAT QLISNMDIDV ILGGGRKYMF RMGTPDPEYP DDYSQGGTRL
DGKNLVQEWL AKRQGARYVW NRTELMQASL DPSVTHLMGL FEPGDMKYEI
HRDSTLDPSL MEMTEAALRL LSRNPRGFFL FVEGGRIDHG HHESRAYRAL
TETIMFDDAI ERAGQLTSEE DTLSLVTADH SHVFSFGGYP LRGSSIFGLA
PGKARDRKAY TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLD
EETHAGEDVA VFARGPQAHL VHGVQEQTFI AHVMAFAACL EPYTACDLAP
PAGTTDAAHP GRSVVPALLP LLAGTLLLLE TATAP
UCSC uc002vsq.4; human.;

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Human ALPP ELISA Kit