Intended UseHuman EXENDIN-4 ELISA Kit allows for the in vitro quantitative determination of EXENDIN-4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalBackground: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EDN4 concentrations in Human serum, plasma and other body fluids. Using Purified Human EDN4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EDN4 and EDN4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EDN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.