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Alternative name
Excitatory amino acid transporter 3 ELISA KIT; Excitatory amino-acid carrier 1 ELISA KIT; Neuronal and epithelial glutamate transporter ELISA KIT; Sodium-dependent glutamate/aspartate transporter 3 ELISA KIT; Solute carrier family 1 member 1SLC1A1 ELISA KIT; EAAC1 ELISA KIT; EAAT3 ELISA KIT
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Catalog
E013813
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species
Human
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GeneEAAT3
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Standard Curve
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SpecificityThis assay has high sensitivity and excellent specificity for detection of EAAT3. No significant cross-reactivity or interference between EAAT3 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human EAAT3 is typically less than 0.117ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
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Intended UseHuman EAAT3 ELISA Kit allows for the in vitro quantitative determination of EAAT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EAAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EAAT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EAAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EAAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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